FastQCFastQC Report
Mon 3 Jul 2023
SRR5443332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5443332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199958654
Total Bases10.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC170783818.54095617186941No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT111861825.594247498785424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA100198485.010959915743381No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGACTAGATCGGAAGAGCACA46942982.3476343264443056No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC44040532.2024818190664557No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGTAGATCGGAAGAGCACACGT39771941.9890081876626355No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGT38722921.9365463422253284No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACACGTC35358281.7682795564327014No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC30638751.532254262923774No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTC23090291.1547532221336116No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGAGATCGGAAGAGCACACGTC19899220.9951667308182621No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTAGATCGGAAGAGCACACG17496260.8749938874863601No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGAGATCGGAAGAGCACACG16460160.8231781756242469No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTC14357970.7180469418442875No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGTC14069830.703636962869334No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTACTAGATCGGAAGAGCAC12844370.6423512932828603No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGAGATCGGAAGAGCACACGT12553630.6278112874274498No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCAGATCGGAAGAGCACACGTCT11732250.5867337954775391Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACGT10908660.5455457806792398No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGCTAGATCGGAAGAGCACACGT9970800.49864308448485556No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGTAGATCGGAAGAGCACACGTCT9609950.4805968537875835Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGT8481550.4241651876692469No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACA8239670.4120686869596552No Hit
TCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC7484230.37428887673948835No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACG7252960.36272298572283845No Hit
AGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT6526190.32637697191140325Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCTG6334380.3167844888573815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGTCGTAGCTCAATTGGTAGAGCACTGGAGATCGGAAGAGCACACGTC5869220.293521679736852No Hit
GAGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC5213220.260714897590779No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAAGATCGGAAGAGCA5020890.2510964091606658No Hit
GCACTCGTAGCTTAACGGATAGAGCATCTGAGATCGGAAGAGCACACGTC4989400.249521583596977No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGTAGATCGGAAGAGCACAC4847800.2424401196459344No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACCTAGATCGGAAGAGCACACG4678160.23395636579950171No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGATCGGAAGAGCACACGTCTGAA4646120.2323540345495624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCGTAGCTTAACGGATAGAGCATCTGAAGATCGGAAGAGCACACGT4609240.23050965326061856No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGCCAGATCGGAAGAGCACACG4590160.22955545599941876No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGAGATCGGAAGAGCACACGTCT4395090.2197999392414394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCTCATCGTCTAGTGGCCCAGGACGCCGTAGATCGGAAGAGCACACGT4239860.2120368343747703No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCCTAGATCGGAAGAGCACA3758900.18798386190377137No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGT3603610.18021775641678403No Hit
TGGCCTATGGTGTAATTGGCAGCACGACTGAGATCGGAAGAGCACACGTC3593950.17973465654554766No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGGCAGATCGGAAGAGCACACGT3499270.1749996776833675No Hit
CGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGTAGATCGGAAGAGCAC3484280.17425002270719425No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACACG3428050.17143794136561852No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGACCAGATCGGAAGAGCACA3417180.170894328984631No Hit
GCGGGTGTAGTTGAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC3260330.16305020736936945No Hit
CGGGTCGTAGCTCAATTGGTAGAGCACTGGTAGATCGGAAGAGCACACGT3213240.16069522052293872No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTAGATCGGAAGAGCACACGTCTG3045140.15228848259800748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGTAGCTTAACGGATAGAGCATCTGAGATCGGAAGAGCACACGTCT3027840.15142330373958207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGATGATCCTATGCTGTCGAGAAAAGCCCAGATCGGAAGAGCACACGTC2904600.14526002960592044No Hit
CCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTCT2820760.14106716281456866Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCTGTAGCTCAATTGGCAGAGCAGCCGGCAGATCGGAAGAGCACACGTC2789680.13951284148972118No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGATCGGAAGAGCACACGTCTGA2764560.13825658178315203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC2742810.13716885691779063No Hit
GAAGTGGATGATCCTATGCTGTCGAGACAAGCCTAGATCGGAAGAGCACA2742680.13716235557376777No Hit
AGGGACGTGGCGCAGCTTGGTAGCGCACTTGACTAGATCGGAAGAGCACA2721130.1360846327761338No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGAGATCGGAAGAGCACA2718350.1359456040347221No Hit
CCCTCATCGTCTAGTGGCCCAGGACGCCGCAGATCGGAAGAGCACACGTC2702150.13513543654879773No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGCAGATCGGAAGAGCAC2614220.1307380274724194No Hit
CTCGTAGCTTAACGGATAGAGCATCTGAAGATCGGAAGAGCACACGTCTG2613700.13071202209632798Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGACGTGGCTCAGTTGGTAGAGCATCACCTAGATCGGAAGAGCACACGT2587620.12940775246466701No Hit
AGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGGCAGATCGGAAGAGCACAC2576010.12882713243308788No Hit
CTGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT2555720.12781242266213694Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACTCGTAGCTTAACGGATAGAGCATCTGAAGATCGGAAGAGCACACGTC2474490.12375008285462853No Hit
CGGACGTGGCTCAGTTGGTAGAGCATCACCAGATCGGAAGAGCACACGTC2462910.12317096313320852No Hit
ACGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC2372770.11866303120844172No Hit
TAGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC2362850.11816692864915965No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGAGATCGGAAGAGCACACGT2336220.11683515333124816No Hit
ATGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT2332250.11663661228685807Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCGGAAGATCGGAAGAGCACACG2098700.10495669769811514No Hit
CCGGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTC2042950.1021686213190853No Hit
GCGGTGTAGTTTAATGGTAGAACATGAGCAGATCGGAAGAGCACACGTCT2042700.10215611873442597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCCAATAGCTCAGTCGGTAGAGCGTCTCCAGATCGGAAGAGCACACGT2002240.10013270043316054No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)