FastQCFastQC Report
Mon 3 Jul 2023
SRR5445272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19669233
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA13406666.816056325124626No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA8058044.0967738803033145No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT5015982.550165530094641No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA3785521.9245895353418203No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1908980.9705411492151219No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1451680.7380460641246154No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG1380590.7019033228189426No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG1304010.662969420312424No Hit
TTGCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1296470.6591360222333021No Hit
GTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA1204060.6121540173935608No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG1052790.5352471039414705No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC1049600.5336252816772266No Hit
TTGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG1027740.522511477697173No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA898670.45689122702446No Hit
TTACTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA896240.4556557950175282No Hit
CTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA803470.4084907632137969No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG767130.39001520801548284No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG764920.3888916258198782No Hit
ATGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA733570.3729530277057575No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA729750.3710109082545313No Hit
TTCCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA691390.35150836842494065No Hit
TGTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA687960.3497645281847035No Hit
TGGTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT658030.33454786976187634No Hit
TGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC597380.30371291041191084No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA594540.30226903102932384No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG537800.27342194787158197No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA531650.27029523723675447No Hit
GGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT506620.2575697791571232No Hit
ATACTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA505080.2567868304778331No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT499250.25382281047766325No Hit
TGGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA495860.25209930656675833No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT449580.2285701735293898No Hit
ATGCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA436480.22191002567309054No Hit
TGATTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT427240.21721233359734976No Hit
AGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT401770.20426317589506415No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCCTGTAGGCACCATCAATAGATCGG386510.1965048662548255No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA378820.19259520694070784No Hit
TATCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA359600.1828236006965803No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT351420.17866482134814307No Hit
GATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT349090.17748023016454173No Hit
TCGAAATCAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC345190.17549743805465115No Hit
ATGTTACCGTCACTTGAGTAAATACCTGTAGGCACCATCAATAGATCGGA332810.16920334412633173No Hit
TCTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA327380.16644268741948404No Hit
CTGGAGATAGGGTTTAATGGCCGGCTGTAGGCACCATCAATAGATCGGAA326820.16615797880883304No Hit
CGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT309040.15711848042066512No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG300450.15275125369657272No Hit
TTTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT294780.14986857901373177No Hit
AGTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA281870.14330502872176054No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG274480.13954789187763447No Hit
AATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT267960.136233070196484No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT263530.13398082172294162No Hit
ATCCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA261130.1327606419630089No Hit
TGTGTATTTATTAGATTAAAAACCACTGTAGGCACCATCAATAGATCGGA260700.13254202642268767No Hit
GTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGA257350.13083885884111496No Hit
TAGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA255600.1299491444328307No Hit
TGCTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT245830.12498199599343808No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC244270.12418887914948183No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA243740.12391942278583003No Hit
AATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC236320.12014703369470482No Hit
TCGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA236220.1200961928713743No Hit
GTGCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA222270.11300389801676557No Hit
AAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATC214990.1093026860783031No Hit
TCTGGAGATAGGGTTTAATGGCCGGCTGTAGGCACCATCAATAGATCGGA211990.10777746137838726No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG207970.1057336602805No Hit
TTGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATA201760.10257644515167419No Hit
TAGTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT200280.10182400096638236No Hit
TTGGGTCGGGCCTTTTTTCCTGGACTGTAGGCACCATCAATAGATCGGAA197450.10038520566612841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)