FastQCFastQC Report
Mon 3 Jul 2023
SRR5445273.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445273.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48569293
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA25481965.246516559341311No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA21980574.525610451031272No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT20783644.279172851043971No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA9163791.8867456028235783No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG4367850.8993027755211509No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT3686770.7590742570619672No Hit
GTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA3507690.7222032241647001No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA2774990.5713465913535122No Hit
TGGTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT2691600.5541773070487149No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG2588510.5329519620555316No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT2545800.5241583401265487No Hit
TTGCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA2540070.5229785823730232No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG2446680.5037503840132077No Hit
CTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA2265160.46637697608651624No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA2176920.4482091184650351No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC2133700.4393104919192462No Hit
GGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT2105280.4334590581748843No Hit
ATGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA1991420.41001626274444636No Hit
TGATTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1771270.3646892698232194No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1730040.3562003671743791No Hit
TTACTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1695720.34913417413755643No Hit
TTGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG1663700.34254153133338794No Hit
AGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1659090.34159237195402453No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1598210.3290577031870734No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG1573090.3238857110808675No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1471440.30295684971160686No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG1447300.2979866311827928No Hit
TGGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA1348840.277714563397083No Hit
TGTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1322190.27222755744045934No Hit
TTCCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1318700.27150899643525794No Hit
CGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1287390.2650625365289958No Hit
ATACTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1146060.23596390418942273No Hit
ATGCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA1085390.22347247261762693No Hit
TCGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCG1055540.2173266141633974No Hit
TGCTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT1026680.21138458820061473No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC1020230.21005658863512797No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG909710.18730147049906615No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCCTGTAGGCACCATCAATAGATCGG900270.18535785563112891No Hit
AAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGAT881290.18145003675470425No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG868480.17881256785022587No Hit
GATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT780780.16075589158771572No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT777370.1600538018949545No Hit
ATGTTACCGTCACTTGAGTAAATACCTGTAGGCACCATCAATAGATCGGA756280.15571155215292098No Hit
TGTAATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAG751450.15471709666434716No Hit
TCGAAATCAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC714360.14708058443428443No Hit
TATCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA692230.142524207630529No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG686540.14135268553322364No Hit
TAGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA684590.14095119729249506No Hit
TGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC673950.13876051273795564No Hit
AGTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA671230.13820048811499067No Hit
TTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT647400.13329409592188216No Hit
TTGTTTTTTTACTTATTCAATTAAGCCTGTAGGCACCATCAATAGATCGG644220.13263936125238635No Hit
TCGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA636360.131021054805142No Hit
ATCCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA627510.12919891586645085No Hit
TCTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA620210.12769590860628752No Hit
GTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG604210.1244016461182583No Hit
AGATTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT588880.12124533087191532No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG583040.12004292506378464No Hit
CTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG576200.11863462785015215No Hit
TAAGGCTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAA550420.11332674741631509No Hit
AATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT539000.11097546756548424No Hit
TGTAATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGA535580.11027131895866799No Hit
AATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC499980.10294158492280298No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT495090.10193477594989905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)