FastQCFastQC Report
Mon 3 Jul 2023
SRR5445274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19715235
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA346522317.576371775431536No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT19630699.95711691998599No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA8332234.226289973211072No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA7875633.994692429484102No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG7125213.6140629315349275No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT3804391.929670125666775No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA2514621.2754704673822048No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG1749340.8873036512118673No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1520080.771017946273529No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1015500.5150838932429667No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA973440.49375013790096844No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC877990.44533580248980037No Hit
TCGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCG775770.39348757445701255No Hit
TGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC699620.35486262273820224No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT656380.3329303454916972No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT618760.3138486556208942No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT527990.26780811894963463No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA467160.23695380755035383No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCCTGTAGGCACCATCAATAGAT451180.2288484007418628No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG449530.2280114845194592No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA444800.2256123246819021No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC418440.21224195400156276No Hit
TGGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATC375620.1905227099753059No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG360040.18262019194800366No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC348500.17676685061070793No Hit
GATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT346350.17567632341181832No Hit
TCGAAATCAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC330260.16751512218850043No Hit
AATTAAGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT305640.15502731770633218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATCTGTAGGCACCATCAATAG304470.15443386802135506No Hit
TTTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT303860.1541244626300422No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAG300450.15239483577040802No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG298950.15163400284095016No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA294920.14958989837047337No Hit
GTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA288050.14610528355355643No Hit
CTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA285880.14500461191560737No Hit
TAAGGCTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAA277660.14083524746217835No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA267560.13571230573716217No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCCTGTAGGCACCATCAATAGATCGG266370.13510871161312557No Hit
CGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCGG262390.13308996824029742No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGCTGTAGGCACCATCAATAGAT235960.11968409202325005No Hit
CTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG228020.11565674971665313No Hit
CAAGATTGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA209230.1061260492203111No Hit
GGAATGCTGCTCAAAATGGGAGGTACTGTAGGCACCATCAATAGATCGGA203110.10302185086812304No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG202780.10285446762364234No Hit
CTTTGAACGCACATTGCGCTCGCCAGTCTGTAGGCACCATCAATAGATCG198930.1009016631047005No Hit
TGGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGAT197270.10005967466276715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)