FastQCFastQC Report
Mon 3 Jul 2023
SRR5445275.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445275.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20063552
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA273527513.633054605684974No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT13720856.838694364786455No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA8920394.44606717693856No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG6138233.0593934713055795No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA5947652.9644053057006055No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT3510371.7496253903595933No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT3482541.7357544666069098No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA3211091.6004593802732436No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA2745271.3682871308131281No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG2172851.0829837109600533No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1851630.9228824487309127No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1369700.6826807137639437No Hit
TCGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCG1266760.6313737467822248No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC1173620.5849512588797836No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1103020.5497630728596811No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT608050.30306199021987734No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA594750.29643305432657185No Hit
TGGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATC517750.2580550044179615No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT477020.23775451126500433No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC462980.23075674735959015No Hit
ACCAGCGGAGGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA419720.20919526113820724No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG412680.20568641086084857No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG404110.20141498374764347No Hit
TGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC385710.19224412506818334No Hit
TACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA375350.18708053289866122No Hit
CGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCGG367990.18341218942687718No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAG362240.18054629608954587No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA360230.17954447946206134No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG354660.17676830104659433No Hit
TAAGGCTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAA347590.1732444982822583No Hit
TGTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAATAGAT322310.16064453592265218No Hit
GTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGATC321220.16010126222914067No Hit
TTTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT317900.1584465203369772No Hit
GATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT316340.15766899101415344No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCCTGTAGGCACCATCAATAGAT315530.15726527386576414No Hit
GTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA314300.15665222189969152No Hit
CTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA295290.14717732931835797No Hit
TGGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGAT293760.14641475248251157No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA290070.14457559658429375No Hit
CTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG283510.14130598609857317No Hit
CAAGATTGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA274100.13661588935000143No Hit
TCGAAATCAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC272010.13557419942391058No Hit
TCTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT258430.12880570698548294No Hit
GTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAATAGATC256620.1279035736045143No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATCTGTAGGCACCATCAATAG255300.12724566417750954No Hit
TGGCCGGAAAGCTATCCAAACTCGGTCATTCTGTAGGCACCATCAATAGA254380.12678712124353653No Hit
GGAATGCTGCTCAAAATGGGAGGTACTGTAGGCACCATCAATAGATCGGA254340.12676718459423336No Hit
AAACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCA234890.11707298887056489No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGCTGTAGGCACCATCAATAGAT233630.1164449844175149No Hit
CTTTGAACGCACATTGCGCTCGCCAGTCTGTAGGCACCATCAATAGATCG225740.11251248034246379No Hit
TATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCG224650.1119692066489523No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA209260.104298580829556No Hit
TAAGGCGCTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGG208470.10390483200581832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)