FastQCFastQC Report
Mon 3 Jul 2023
SRR5445276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25574066
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA459013317.948389591236687No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA266649110.426543045599397No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG21480498.399325316514004No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT7100062.776273432625066No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT5790952.2643837706526604No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT4485341.753862682609797No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA4273571.6710561394500196No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA1270010.49660073607380223No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA1055500.4127227950377543No Hit
CTTTGAACGCACATTGCGCTCGCCAGTCTGTAGGCACCATCAATAGATCG944870.36946412823052854No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT930670.36391162828781315No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT913110.35704529737273694No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT872600.3412050316910889No Hit
TCGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCG739070.2889919811734278No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA735300.2875178315407491No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC723630.28295461503853164No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG679640.26575359584979563No Hit
TGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGATC620260.2425347615822998No Hit
TCTTTGAACGCACATTGCGCTCGCCAGTCTGTAGGCACCATCAATAGATC609580.23835865599158146No Hit
GATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT585740.22903671242578322No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA573440.22422715261624804No Hit
GTGAATCATCGAATCTTTGAACGCACACTGTAGGCACCATCAATAGATCG552610.21608218262985637No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA540990.21153851718377514No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGAACTGTAGGCACCATCAATAG522500.20430853662456333No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT520130.2033818165637017No Hit
ATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG518140.20260368452947605No Hit
GCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGATCGG511530.2000190349082543No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA500620.1957529944593089No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA442710.17310896124222092No Hit
CGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCGG437660.1711343045724524No Hit
TTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT430930.16850273241650351No Hit
GGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGATCG428510.16755646129950552No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAATA409390.16008013743297606No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAG402740.15747984696684525No Hit
TATGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATC378150.14786463755900214No Hit
AATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT374570.1464647819396415No Hit
GGAATGCTGCTCAAAATGGGAGGTACTGTAGGCACCATCAATAGATCGGA328310.12837614480231652No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG321140.125572523352368No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA314370.12292531035151No Hit
TTGGGTCGGGCCTTTTTTCCTGGAGAACCGCTGTAGGCACCATCAATAGA295670.11561321535652562No Hit
GTGTAGCGGTAACATCGTTGACTGTAGGCACCATCAATAGATCGGAAGAG293410.1147295076191639No Hit
TGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCGG290330.11352516256116646No Hit
TGGAAATCTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC287900.11257498123294121No Hit
TTGGATATAAAGCTTATGTTTATATATCTGTAGGCACCATCAATAGATCG276110.10796484219599652No Hit
GTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAATAGATCGG268950.10516513095727524No Hit
TCGTCCAACTTCTGGTTTTAAGGTCCTCTGTAGGCACCATCAATAGATCG256850.10043377537228536No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)