FastQCFastQC Report
Tue 4 Jul 2023
SRR5445277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5445277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24215759
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA379165015.65777888688106No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG370637215.305619782555649No Hit
TCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGA13092525.406611454962036No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT12972385.356999134324057No Hit
ATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA9866254.074309626223155No Hit
AACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA4628411.911321466322819No Hit
TGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGAT4174071.7236998435605506No Hit
GGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATCG3774091.5585264124903127No Hit
TATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT1878620.775784066896272No Hit
CTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAGAT1691340.6984459995658199No Hit
CTGTGTTCAGGTCGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1320120.545149132017708No Hit
GGCGCCGGTGAAATACCACTACTCCTATCTGTAGGCACCATCAATAGATC1278820.5280941225092305No Hit
CTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAATA1273540.5259137241991878No Hit
TCGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCG1171070.4835983047237958No Hit
ACCAGCGGAGGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA999830.412884023168549No Hit
CGGCCCGAGTTGTAATTTGTAGAGGACTGTAGGCACCATCAATAGATCGG923950.3815490565461937No Hit
TTGTTTTTTTACTTATTCAATTAAGCGGGGCTGTAGGCACCATCAATAGA901380.3722286796792122No Hit
GCCAAGGATGTTTTCATTAATCAGGAACGACTGTAGGCACCATCAATAGA873310.3606370545726029No Hit
AAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA778790.32160462118903643No Hit
TACTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAA681980.2816265226293341No Hit
TTTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAAT665020.2746228189667728No Hit
TTCTGGCGAGCATGCCTGTTCGAGCGTCATTTCTGTAGGCACCATCAATA652620.2695021865719757No Hit
TCTGGTGATTCATAATAACTTCTCGAATCGCATCTGTAGGCACCATCAAT633680.2616808335431485No Hit
GAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAGA554420.2289500816389856No Hit
CTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG516120.21313393480666865No Hit
TGAATGGCTCAGTGAGGCTTCCGGACTGGCCCTGTAGGCACCATCAATAG515020.2126796851587431No Hit
TGGCGCCGGTGAAATACCACTACTCCTACTGTAGGCACCATCAATAGATC495990.20482116624963106No Hit
CTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA438550.18110107554340954No Hit
TGTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAATAGAT369510.15259071582270042No Hit
ACTGGTGATTCATAATAACTTCTCGAATCGCACTGTAGGCACCATCAATA356450.14719753363914798No Hit
GTTACGATCTGCTGAGGGTAAGCCCTCCCTGTAGGCACCATCAATAGATC351590.14519057610376782No Hit
ATGGATATAAAGCTTATGTTTATATACTGTAGGCACCATCAATAGATCGG334290.13804646800457504No Hit
GCATAAGCATGGCAGGAAAGCTAAGTTAGTCACTGTAGGCACCATCAATA310890.12838333913052238No Hit
TGGCCGGAAAGCTATCCAAACTCGGTCATTCTGTAGGCACCATCAATAGA304160.1256041571936688No Hit
ACTGGTGATTCATAATAACTTCTCGAATCCTGTAGGCACCATCAATAGAT271830.11225334708691145No Hit
GTTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATA262930.10857805448096837No Hit
GTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAATAG254350.10503490722714906No Hit
CATTCTGGCGAGCATGCCTGTTCGAGCGTCATTCTGTAGGCACCATCAAT252760.10437831000878395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)