FastQCFastQC Report
Tue 27 Jun 2023
SRR544881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR544881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10405389
Total Bases520.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTA2454872.3592294339019904No Hit
AAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACG1584951.5232011028131673No Hit
TAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAAC1546041.485807017882753No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC1114391.0709738963146884RNA PCR Primer, Index 7 (100% over 50bp)
ACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAAT878830.8445912017321024No Hit
GTAGTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTT851700.8185181736117698No Hit
AGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATTCT799660.7685056272283526No Hit
AGTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGG635600.6108373266967723No Hit
TAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATTC635200.6104529105062771No Hit
GTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGA618620.5945188594102537No Hit
TACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAA593560.5704351850757333No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTTGGAATTCTCGGGTGCCAA592530.5694453133852083No Hit
AACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAAGG592260.5691858324566241Illumina Small RNA Adapter 2 (100% over 21bp)
ACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAG581860.5591910115037506No Hit
AAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCG558780.5370101973121811No Hit
CTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATT540740.5196730271208505No Hit
AGTAGTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCT523860.5034506638819558No Hit
TGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATTCTCGGGTGCC423060.40657778387718135No Hit
AGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGG372930.3584008248033783No Hit
TCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCC343400.33002129954007486No Hit
TAAAACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCA309010.2969711175622555No Hit
CTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCA294560.2830840826806187No Hit
AAACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGT286810.2756360189897754No Hit
CAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTC283090.27206094841817063No Hit
AAAACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAA281660.2706866605371505No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTT269000.25851988810797943No Hit
TGCGCCCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGG265240.2549063759173251No Hit
AGAACATGAAACCGTAAGCTCCCAAGCAGTGGGAGGAGCCCTGGGCTCTG258370.24830402784557118No Hit
AGAGTAGTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCC257220.24719883129789766No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCAATCCATCCCGAACTT248660.23897232482130173No Hit
TAGTACTAGGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTG245940.2363582947259348No Hit
GGATGCGATCATACCAGCACTAATGCACCGGATCCCATCAGAACTCCGCA224260.21552293720109839No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCTGGAATTCTCGGGTGC219140.21060240996276064No Hit
AAAACGACTCTCGGCAACGGATATCTCGGCTCTTGGAATTCTCGGGTGCC203190.19527381436676708No Hit
TAAAACGACTCTCGGCAACGGATATCTCGGCTCTTGGAATTCTCGGGTGC201650.19379381203336077No Hit
CCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCT195430.18781614027116142No Hit
GCGCCCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGA183480.1763317065801192No Hit
TTGCGCCCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATG183280.17613949848487165No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGGAATTCTCG178520.17156494581797951No Hit
CCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGT176760.1698735145798009No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTGGAATTCTCGGGTGCCAAG172230.16552000122244348No Hit
ATTACGATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCAT169120.1625311653413438No Hit
TTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGC167390.16086856531745233No Hit
TGAGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCA161710.1554098554124214No Hit
AGTTCTACAGTCCGACGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGT159160.1529592021980149RNA PCR Primer, Index 1 (100% over 30bp)
ACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGTGCCAAGGA157820.15167140795985617RNA PCR Primer, Index 1 (100% over 22bp)
AGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAAG156020.1499415351026281No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCTGGAATTCTCGGG153870.14787529807871672No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGATGGAATTC149890.1440503569832901No Hit
ACGACTCTCGGCAACGGATATCTCGGCTCTTGGAATTCTCGGGTGCCAAG149310.14349295350707217No Hit
TACGATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCC148380.14259918586417095No Hit
GAGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCGGGTGCCAA148240.14246464019749766No Hit
ACGATAGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCCT146880.1411576251498142No Hit
GGATGGGTGACCTCCCGGGAAGTCCTCGTGTTGCATCCCTTGGAATTCTC146820.14109996272123992No Hit
CGCCCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAA146530.14082126098313097No Hit
CTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTG145660.13998515576880402No Hit
ACTCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTACTAGGATGGGTGACC143300.13771710024488273No Hit
AACGACTCTCGGCAACGGATATCTCGGCTGGAATTCTCGGGTGCCAAGGA143130.1375537233639223RNA PCR Primer, Index 1 (100% over 22bp)
AGGTGTCAAGTGGAAGTGCAGTGATGTATGCAGCTGAGGCATCCTAACAG140080.13462254991139685No Hit
AGAACTCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTACTAGGATGGGTG137540.13218150710175278No Hit
CCCAAGCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTC136780.131451116339812No Hit
CGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGC128030.12304201217273089No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGTGGAATTCTCGGGTGCC125540.12064902138689865No Hit
AGAGTGGCCTTGCTGCCACGATCCACTGAGATTCAGCCCTTTGTCGCTAA124520.11966876010113606No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGTGGAAT123090.11829447222011594No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATTGGAATTCTC121130.11641083288668977No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACTGGAATTCTCGGGTGCCAA117790.11320095769605537No Hit
TATTCTGGTGTCCTAGGCGTAGAGGAACAACACCTGGAATTCTCGGGTGC117520.11294147676747116No Hit
GCCTTCTGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGG115380.11088485014832218No Hit
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATGGAATTCTCGGGTG115350.11085601893403504No Hit
ATGCAGCTGAGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCG114490.1100295241244705No Hit
TAAAACGACTCTCGGCAACGGATATCTCGGCTCTCGCTGGAATTCTCGGG113380.1089627691958465No Hit
GAACTCCGCAGTTAAGCGTGCTTGGGCGAGAGTAGTACTAGGATGGGTGA108720.10448432057657815No Hit
TACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCC108000.1037923714336869No Hit
GACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACGTAGCG106730.10257185002886485No Hit
TGCAGCTGAGGCATCCTAACAGACCGGTAGACTTGAACTGGAATTCTCGG105530.10141860145737945No Hit
GAAGGTCACGGCGAGACGAGCCGTTTATCATTACGATAGGTGTCAAGTGG105260.10115912052879522No Hit
AACGACTCTCGGCAACGGATATCTCGGCTTGGAATTCTCGGGGGCCAAGG105080.10098613324307242Illumina Small RNA Adapter 2 (95% over 21bp)
AACGACTCTCGGCAACGGATATCTCGGCTCTCGCATCGATGAAGAACTGG104560.10048639219542874No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)