FastQCFastQC Report
Sat 1 Jul 2023
SRR5453820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5453820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124471436
Total Bases10.7 Gbp
Sequences flagged as poor quality0
Sequence length86
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT59191394.755419548626402No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA45988283.6946854216416365No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA41792463.357594428331332No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG25818362.074239747663874No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA24020221.9297776881115118No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC21693501.742849660704485No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA21662711.740376000803911No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG19593711.574153125380509No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA18880441.516849215108276No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA15824291.2713189875948727No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA15507081.2458344258195913No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC11475640.9219496752652552No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC9299010.7470798360517027No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC8596470.6906379709478084No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA8233880.6615075927942216No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA7783580.6253306180222745No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT7359090.5912272113579536No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA7358080.5911460682433197No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG6862930.5513658571433209No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCA6767810.5437239432185871No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC6544590.5257905114873103No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA6481040.5206849224427683No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA6264350.503276109066501No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT6022720.4838636231367975No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT5618030.4513509428781716No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT5410050.43464188844097534No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA5350060.4298223087905887No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA4961920.39863925085591523No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4820040.38724065174278216No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC4774900.38361411689666697No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG4699300.37754043425673983No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA4634150.37230630166426293No Hit
TTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4510820.3623980043100009No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC4314750.34664579590774547No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC3879560.3116827542666094No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3874700.31129230323975693No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA3845180.30892067478035684No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3840440.3085398645195995No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT3824090.30722631013913904No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC3797730.3051085551869105No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCAC3773130.30313219813741044No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA3571970.28697106057328686No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAAT3484600.2799517794588631No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA3268460.2625871529271985No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3267470.26250761660691374No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATA3221010.2587750333337522No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT3216100.2583805653210267No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA3198400.25695855232199616No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG3148250.25292951549140963No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3144470.25262583135941324No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA3134730.25184332251135916No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT3128300.25132673812809553No Hit
TATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCA3119030.2505819889472473No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC3007120.24159117116637102No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCA2928160.2352475470757805No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA2889760.23216250192534135No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA2860690.22982702633879792No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC2853310.2292341192239479No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC2832810.22758715501603113No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA2825530.22700228187292706No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGAT2787410.2239397318433765No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2761670.22187178751597275No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATA2721280.21862686632778947No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC2691130.21620462384638997No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGCACCATC2684990.2157113379811895No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATCA2569940.2064682534874909No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2527420.2030522087011192No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCA2467990.1982776192925098No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT2427840.19505197963651677No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC2355050.18920405160265044No Hit
GCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG2353490.18907872164341383No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT2247150.1805353960887862No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT2241250.18006139175577598No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCTGTAGGCACCAT2171010.174418330001431No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2165060.17394030868254784Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTAGGCACCATCA2164270.17387684030575498No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC2114470.16987592237627916No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2112830.16974416523964583No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC2102360.1689030083978464No Hit
CAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC2091200.168006417151No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1990010.15987684114128803No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT1944670.15623423835168093No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA1917480.15404980143396113No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT1858880.149341893990843No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1823080.14646573210579814No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCA1775260.14262388681689186No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG1767000.14196028075067763No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACCATCAATAG1761580.14152483948204791No Hit
AAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCA1747010.14035428979866515No Hit
CCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC1696320.1362818695206505No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA1692870.13600469749541572No Hit
TCGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGGGTCTGTAGGCAC1679890.13496188796279335No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1668050.1340106657080746No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC1651180.1326553346745353No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT1564150.12566336906404776No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1563200.12558704633246137No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA1552330.1247137536036782No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA1532830.1231471291132208No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGG1527640.12273016597960677No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTGTAGGCACCATCAATA1521490.12223607671723175No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCA1520280.12213886565910592No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1499440.12046458594725298No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1492140.1198781060097997No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCCTGTAGGCACCATCAAT1467100.1178663994846175No Hit
GAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC1465450.11773383895080956No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCA1397060.11223940567376438No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1395920.11214781839586072No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAA1387920.11150510065618588No Hit
AAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC1376340.11057476672800659No Hit
TCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGAAGA1371460.11018270890680493No Hit
TGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATC1367070.10983001754715836No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAAT1332970.10709043318179441No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGCACCAT1273670.10232628793645476No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAAT1268170.10188441949042831No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT1264540.10159278631605086No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1264050.10155341985449579No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)