FastQCFastQC Report
Sun 2 Jul 2023
SRR5453821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5453821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91804699
Total Bases7.8 Gbp
Sequences flagged as poor quality0
Sequence length86
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT34534713.761758425894953No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA32660453.5576011201779547No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA32585723.5494610139727163No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA14646241.5953693176424446No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA13811931.5044905272223594No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA12253891.3347780814574643No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG11430791.2451203614316082No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC10935181.1911351073652559No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC10665991.161813078870832No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA10062141.096037578642897No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA8159180.8887540712921459No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG7269510.7918450884523895No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA7265260.7913821491860673No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA6783310.7388848363851179No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA6542630.7126683134160703No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA6433440.7007745867126038No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC6268720.6828321500188134No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6223220.6778759766970098Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAAT6165330.6715701992552691No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC5825310.6345328794117608No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA5532760.6026663188558572No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATA5448330.5934696218545414No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA4982520.542730388996755No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG4668130.5084848652463857No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT4598190.5008665188260135No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA4556470.4963220891340213No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT4269630.4650775010982826No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT4121440.44893562583327024No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA3944620.4296751738165385No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA3915040.42645311652293527No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3858560.4203009259907273No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATCA3807330.41472060161103513No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC3527200.3842069129816547No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC3367290.36678841461045475No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3324260.36210129069754915No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA3255730.35463653118670974No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCA3031130.33017155254765335No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC2972830.3238211150825733No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGAT2711250.2953280201920819No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT2707210.2948879555718602No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA2640640.28763669275796006No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG2597570.2829452117696067No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG2574120.2803908762883695No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC2570550.2800020073046588No Hit
GGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAAT2458740.2678228921593654No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATC2438800.2656508900486673No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA2433770.26510298781111413No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2398610.26127311849255125No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT2387450.260057494442632No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGCTGTAGGCACCATCAATAG2345060.2554400837368902No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA2331230.2539336249008343No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT2277240.24805266231524817No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC2252650.2453741501837504No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA2241690.2441803115110698No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC2217080.2414996208418482No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC2166900.2360336696926592No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2129440.2319532685358513No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCCTGTAGGCACCATCAAT2091510.22782167174253248No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2087350.2273685358959676No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTGTAGGCACCATCAATA2078880.22644592516990877No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2075680.22609735913408963No Hit
GGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATA2064650.2248958955793755No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC2059110.22429244062986362No Hit
TTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2052090.22352777388878536No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCTGTAGGCACCATCA2019170.21994190079529588No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1985210.2162427437401652No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCA1948220.21221353821986821No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA1921790.20933460061777448No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT1910180.20806995946906814No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA1899730.20693167350834624No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCCTGTAGGCACCATC1854970.20205610608232594No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA1835620.19994837083448203No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1830530.19939393298375718No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1811320.19730144749998038No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA1769920.19279187441157017No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG1745380.19011880862438207No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT1618220.1762676657760187No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCAT1616900.17612388228624332No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1609260.1752916808757251No Hit
CCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC1600320.17431787451315536No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCTGTAGGCAC1583540.1724900813628287No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT1573500.17139645542544615No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1573210.17136486662845005No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATC1467860.15988941916796656No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1412600.15387011943691467No Hit
TCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGC1331960.14508625533427216No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1281460.13958544758150127No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCA1241020.13518044430383677No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCA1223800.13330472332358498No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT1221320.13303458464582515No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1219740.13286248016563945No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC1217910.13266314396390536No Hit
CGGGGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCA1203170.13105756166141344No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1189920.12961428041934978No Hit
TATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCA1189090.1295238711038092No Hit
GGGGCGGGTCGGGGGTCGGCGCGCGGCGCGGGCTGTAGGCACCATCAATA1176580.1281611957575287No Hit
TCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCAATAGATCGGAAGA1174240.12790630684383597No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC1172390.12770479210437802No Hit
GCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC1168460.1272767094416376No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC1150610.12533236452308394No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA1120470.12204930817321234No Hit
TTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCA1114390.12138703270515598No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA1102770.1201213022875877No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAG1102270.12006683884449094No Hit
TGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCAT1065870.11610190018704815No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCA1054550.11486884783533792No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATCG1050810.1144614612809743No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1036500.11290271753954555No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT1032210.11243542119777551No Hit
CGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATCG1029490.11213914006732922No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGTAGGCACCATCAATA1023700.11150845339626897No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGG1021730.1112938674304678No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCA1008200.10982008666026996No Hit
CAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC1007590.10975364125969193No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT987910.10760996013940419No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCATC983600.10714048525991028No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT979690.10671458113489375No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT961500.10473320107503428No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA950520.10353718386462984No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT946470.10309602997554625No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAA938980.10228016759795705No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGGCTGTAGGCA922780.10051555204162262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)