FastQCFastQC Report
Tue 4 Jul 2023
SRR5453822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5453822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145334412
Total Bases12.4 Gbp
Sequences flagged as poor quality0
Sequence length86
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT41832942.878391939274506No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41038902.8237565649627427Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA39704192.73191940254315No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG31620842.1757297232537054No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC25036651.7226924893740927No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA20795661.4308834166542745No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAA17813121.2256642975925067No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA12653650.8706575287895341No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA10960920.7541861455358556No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG10835380.7455481362528236No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATC10148260.6982695880725069No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA9784160.673217021719536No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA8280780.5697742114923202No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAAG8158600.5613673931539352No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC8153010.5609827629811445No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAA7990090.5497727544389143No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA7973200.5486106071010904No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT7599320.5228851099628077No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA7215080.49644677407853005No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG6991760.4810808330789545No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATC6643010.457084451547511No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT6640770.45693032425107966No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA6508880.4478553916053962No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATCA6403400.44059764730737No Hit
TTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA6033820.4151680195327725No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG5949960.4093978788726238No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGCCTGTAGGCACCATC5665030.38979274915289847No Hit
AAATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAAT5558360.38245312472864307No Hit
AATAAGTGGGAGGCCCCCGGCGCCCGGCCCCGCTGTAGGCACCATCAATA5553850.38214280593091743No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGAT5429930.3736162637104831No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA5250710.3612847038593998No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCCTGTAGGCACCATCAATAGA5249990.3612351629426897No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA5141790.3537902640704254No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4940700.3399538988742735No Hit
TCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA4352900.2995092449267968No Hit
CTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATCG4176180.2873497021476235No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGA4084240.2810236023110617No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTGTAGGCACCATC4019260.2765525345779773No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA4013620.2761644640637484No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGG3999350.2751825906172861No Hit
GGGGCGGCGGGTAACCCCGCGGGGGCTGTAGGCACCATCAATAGATCGGA3920840.26978056649102483No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAAT3878860.2668920558195123No Hit
TAGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCA3818410.2627326830207288No Hit
TGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3812140.26230126420437855No Hit
CCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGA3807660.26199300961151584No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAA3631830.24989470490994248No Hit
TATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGGAAGAGCA3612980.24859769618774114No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA3479870.23943881921096566No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC3446690.23715580863257632No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCAC3437760.23654136365171383No Hit
CCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA3378440.2324597425694336No Hit
CGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAATA3369850.23186869191035084No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT3283910.22595543304637306No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA3251980.2237584310039387No Hit
TAACCCCGCGGGGGCGGAGCGGGAGGAACCTGTAGGCACCATCAATAGAT3190330.21951649001063833No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC3130780.21541904335774242No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3024730.2081220791673207No Hit
TCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGATCGG2980150.20505467074102174No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCA2952010.2031184465796029No Hit
TTGGGGAAGGTGGCTCGGGGGGGGCGGCGCGTCTCTGTAGGCACCATCAA2951680.2030957403261108No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCGCTGTAGGCACCATCAATAGAT2935810.20200377595362617No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCA2882210.19831572993187602No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2730040.18784539479885878No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCGTCCCGCGCGCTGTAGGCACCA2718060.18702108899026612No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2683640.18465275794420938No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGTAGGCACCAT2651880.18246745306266487No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA2595730.1786039496275665No Hit
TCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGGAA2590480.17824271377655554No Hit
TCGGGCCCCCGGTGGGGCGGGGGGCCCGGACACCTGTAGGCACCATCAAT2564920.1764840112333478No Hit
CCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGATCGG2560070.17615029811384245No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAAT2525810.17379297616038794No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2496900.17180377074082084No Hit
TGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA2468270.16983383123330761No Hit
TCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATCGGA2392680.16463272304703722No Hit
CCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGG2369710.16305223019032822No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT2353630.16194581638380318No Hit
CTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATCGGAA2338900.16093229179610952No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCTGTAGGCACCATCAA2323860.159897437091499No Hit
TCGAGGTGCGTGGCGGGGGCGGGCGGGCCTGTAGGCACCATCAATAGATC2235470.15381560149704945No Hit
CTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA2213490.1523032274008168No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA2208590.15196607393987321No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCGGA2086370.1435565033283377No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATA2065050.1420895417390893No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCCTGTAGGCACCATCAA2061860.14187004795533215No Hit
TCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAG2008890.1382253502357033No Hit
TCGCGTCTCTCGGGGTCGGGGGTGCTGTAGGCACCATCAATAGATCGGAA1973080.1357613776976646No Hit
TCAACTTCTTAGAGGGACAAGTGGCGCTGTAGGCACCATCAATAGATCGG1952040.13431368202047014No Hit
CCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG1951210.13425657235259603No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1919490.13207401974420208No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT1918710.13202035041776616No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGGAA1910690.13146851965107892No Hit
CTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1865130.12833368053259125No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCTGTAGGCACCATCA1848430.12718460649223254No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAG1842000.12674217858328007No Hit
GGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGATCGGAAGA1820810.125284161881771No Hit
TAGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAA1813040.12474953282227473No Hit
TATCAGATCAAAACCAACCTGTAGGCACCATCAATAGATCGGAAGAGCAC1803880.12411926227079653No Hit
TTGGGGAAGGTGGCTCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG1789830.1231525263266624No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGA1773980.12206193809075307No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1761060.12117295386312224No Hit
GCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATCG1725660.11873719212487681No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1699200.11691656343578148No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA1690530.11632000823039762No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATAGA1673850.11517231032661418No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACCTGTAGGCACC1646310.11327737026245374No Hit
GCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCTGTAGGCACCATCA1643210.11306406909328535No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGCTGTAGGCACCATCAATAGAT1641830.11296911566959103No Hit
CGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGATC1632170.11230444170373084No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1595780.10980056120500903No Hit
AGCGGGCCCCCGGTGGGGCGGGGGGCCCGGACCTGTAGGCACCATCAATA1592080.10954597593858224No Hit
TCTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGTAGGCACCATCAA1553570.10689622496288077No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATAGATCGGA1549980.10664920844761805No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA1536060.10569141739122322No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT1527950.1051333940099472No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1526950.10506458718118322No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATAGATC1526280.10501848660591133No Hit
GCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCTGTAGGCACCATCAAT1522210.10473844281284188No Hit
TCCTCCCGACCCCTACCCGCGCGTCGCTGTAGGCACCATCAATAGATCGG1488640.10242859757123453No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1483700.10208869183714041No Hit
ACCTCACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGAT1482470.10200405943776068No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1482410.10199993102803484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)