FastQCFastQC Report
Sat 30 Sept 2023
SRR5483532.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5483532.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31667620
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAA7941372.5077255568937606No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAA5773291.823089325942398No Hit
ACGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAA2506570.7915245919964935No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAATAGAACAAAAAAAAAAAA1836850.5800404324669804No Hit
ACGTAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA1788050.5646303700751746No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAATAAAAAAAAAAAAAAAAA1522340.48072447503159377No Hit
ACGTTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAAA1272660.40188053285974756No Hit
ACGTTTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAATCG1167580.3686983739226377No Hit
ACGTTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAA1116160.35246096801717336No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAATAGAGTAAAAAAAAAAAA1026180.324047086582446No Hit
ACGTTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAA953230.3010109379864985No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAAAAAAAAAAAAAAAAAAAA928550.2932174883998229No Hit
ACGTTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAAAAAAA850890.2686940161590925No Hit
ACGTATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAA850840.26867822716074025No Hit
ACGTAGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAA730450.2306614769281683No Hit
ACGTTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAAA720940.2276584094415684No Hit
ACGTAGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAA715770.2260258270119447No Hit
ACGTTTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAATCGT642430.20286652422884954No Hit
ACGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTAAAAAAAAAAAAA617430.1949720250527195No Hit
ACGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATAAAAAAAAAAAAAAA546170.17246954460107833No Hit
ACGTAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAA543360.1715822028936813No Hit
ACGTAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAT484990.15315012621725282No Hit
ACGTCTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAA478070.15096492884530002No Hit
ACGTTTTGCTCGAATATATTAGCATGGAATAATAGAATGAAAAAAAAAAA472160.14909866924006288No Hit
ACGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCAAAAAAAAAAAAA443980.14019998976872908No Hit
ACGTTTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAAAA441810.13951474724024096No Hit
ACGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN441070.13928107006462753No Hit
ACGTGTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAA422360.1333728268812118No Hit
ACGTAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAT421280.13303178451680298No Hit
ACGTTTTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAAA401590.12681407696568292No Hit
ACGTTTTGCTCGAATATATTAGCATGGAAAAAAAAAAAAAAAAAAAAACG383260.12102583016974436No Hit
ACGTAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAA361750.11423340307860205No Hit
ACGTTATTCCAGGGGGCATGCCTGTTTGAGCGTCAAAAAAAAAAAAAAAA358810.11330500997548916No Hit
ACGTCTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAA350330.11062719585494583No Hit
ACGTAAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAA344650.1088335656421291No Hit
ACGTTTTGCTCCACACAATATAGATGGATACGAATAAGGCGCCAAAAAAA323450.10213903034077079No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)