FastQCFastQC Report
Sat 30 Sept 2023
SRR5483533.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5483533.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33068533
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA7608242.300749174449317No Hit
TCGATTTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAA3136950.9486208535467842No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAA2737240.8277476354938393No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAT2439420.7376861864419567No Hit
TCGAAAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAA1895010.5730553574904578No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAT1775490.5369122361732829No Hit
TCGATTTCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAA1654290.5002610790143004No Hit
TCGAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAA1412740.4272158066401071No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATC1171060.35413122196863106No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAA986720.29838638442170995No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAATC923010.2791203347303009No Hit
TCGATTTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAA915930.27697932653982565No Hit
TCGAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAT914600.2765771314983946No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAAAA879710.2660263157122815No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAC840980.2543142751448938No Hit
TCGATTTGCTCGAATATATTAGCATGGAATAATAGAAAAAAAAAAAAAAA820830.24822086906606955No Hit
TCGAAAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAA759070.22954450383390157No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAATC733390.22177881310912703No Hit
TCGATCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAA712600.215491869566757No Hit
TCGATTTGCTCGAATATATTAGCATGGAATAATAGAATAAAAAAAAAAAA709930.21468445546102694No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAATCG595140.17997169696037013No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAGAAAAAAAAAAAAAAAAAAAA581310.1757894733340605No Hit
TCGAAAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAT569910.17234208726465125No Hit
TCGAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAATC544710.16472154963753607No Hit
TCGAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATC523750.1583831977064117No Hit
TCGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN518210.15670788903759353No Hit
TCGAAAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAT513610.15531683851835823No Hit
TCGATCCACGTTCTAGCATTCAAGGTCCCAAAAAAAAAAAAAAAAAAAAT509380.1540376768452353No Hit
TCGAAGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAA489000.14787471824044932No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAATCG480520.1453103468484677No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAC468870.14178736020736088No Hit
TCGATAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA462210.13977336097733759No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAAAT456470.13803757185116133No Hit
TCGAAAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAA436580.1320227903669026No Hit
TCGAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAA429370.12984246987914463No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTGTAAAAAAAAAAAAAAAAAAA424700.128430251199834No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTTAAAAAAAAAAAAAAAAAAAA411590.12446575722001335No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAATG408440.12351319001662397No Hit
TCGAAGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAA390060.11795503598541852No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAAAC389270.1177161381788542No Hit
TCGAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAATCG387240.11710226153666993No Hit
TCGATTTTCCACGTTCTAGCATTCAAGGTCTCAAAAAAAAAAAAAAAAAA384710.1163371837510905No Hit
TCGAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATCG381930.1154965053938135No Hit
TCGAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAC373480.1129412060704356No Hit
TCGAAAGAGGTGCACAATCGACCGATCCTGGAAAAAAAAAAAAAAAAAAA368540.11144733877369159No Hit
TCGATTTCCACGTTCTAGCATTCAAGGTCCAAAAAAAAAAAAAAAAAAAA358650.10845658015733567No Hit
TCGAAAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAACG338600.10239341430719046No Hit
TCGAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAAAAAAAAAAAAA330730.10001350831015093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)