FastQCFastQC Report
Sat 30 Sept 2023
SRR5483534.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5483534.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24523690
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAA5125552.090040283497304No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAT4699771.9164204081849021No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAC2331230.9506032738140142No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATC2286620.9324126997201482No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAATC2142670.8737143553845281No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAA1376630.5613470077300765No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAC1238240.5049158589103027No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATCG1132360.46174127955458577No Hit
TGACAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA973040.3967755260321755No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAT845880.3449236228316375No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAATCG813820.33185054940753206No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAACG716650.29222763784732236No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAT656570.26772887766889897No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATG536600.2188088334178095No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAA518370.21137520495488238No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAACG517920.21119170891493083No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAATC508070.20717518448487973No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAAA469150.19130481587395698No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAAT433170.1766332880573845No Hit
TGACATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAAA397650.16214933397054032No Hit
TGACACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAA394080.16069359872025785No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAAT375340.153052008078719No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAACC361020.14721275631848224No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAT352490.14373448693895577No Hit
TGACAATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAA344420.14044379128915754No Hit
TGACAGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAA342120.13950592264051617No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAAA339010.13823776111996197No Hit
TGACAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAATCG330510.13477172480976557No Hit
TGACCTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAA326560.13316103734796841No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAAA319850.13042490750780164No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAAC318920.1300456823585684No Hit
TGACTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAAAA287440.11720911494151166No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAA275840.11247899480053777No Hit
TGACCTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAA274040.11174501064073147No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAATC266710.10875606403440918No Hit
TGACAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAT257890.10515954165135834No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAATCG255050.10400147775477507No Hit
TGACGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAAA253230.10325933821541539No Hit
TGACTTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAAA251020.10235816877476431No Hit
TGACAGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAA250950.10232962494632741No Hit
TGACTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAAAT246560.1005395191343554No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)