FastQCFastQC Report
Sat 30 Sept 2023
SRR5483539.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5483539.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16691481
Total Bases834.5 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAA4695732.813249465400943No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATC3921572.3494440067960416No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAA3175231.9023057330862372No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAT2515931.5073138207448458No Hit
TGACAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAA2418781.449110477374656No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATCG2195431.3152997028843636No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAC2172831.3017598618121424No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAA1964921.1771993150278277No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAT1716761.0285246707586941No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAT1432050.8579526286493092No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAAC1406930.8429030353867341No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAATC1406840.84284911566565No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAACG1244570.7456318585510776No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATG1086920.651182480452154No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAA810230.4854152845993714No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAAA725010.4343592998128806No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAT697420.4178299097605539No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATC694620.4161524073268274No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAACC682330.40878936985879205No Hit
TGACACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAA671620.40237292304978806No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAAA658840.39471632265585066No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAC636970.3816138304324224No Hit
TGACCTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAAAA627700.3760600991607635No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAAAG620000.3714469674680156No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAATCG603090.36131605098433145No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAAT527010.3157359134279337No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAATCGT521890.31266848040626233No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAATC518340.31054164696350195No Hit
TGACAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAAT484650.29035769803769956No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAAA484040.289992242150352No Hit
TGACAAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAA413410.2476772432596005No Hit
TGACAAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAA397370.2380675507463957No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAAT391340.23445492943376325No Hit
TGACATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAAA375040.22468946883742671No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATT365880.21920163944709278No Hit
TGACAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAAACG359910.21562496461518302No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAAC343000.20549404813149896No Hit
TGACAATATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAA332780.19937116424839715No Hit
TGACAGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAA328730.1969447767996141No Hit
TGACAGAGGTGCACAATCGACCGATCCTGTAAAAAAAAAAAAAAAAAAAA328620.19687887491828915No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAATGG326790.19578250725624646No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATA326660.19570462321468057No Hit
TGACAGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAA315720.18915038156290626No Hit
TGACAAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAA315380.18894668483881089No Hit
TGACAATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAA307830.18442341934786974No Hit
TGACGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAATCG305530.1830454709201658No Hit
TGACAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAATCG298770.17899550075874032No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAATC297650.17832449978524972No Hit
TGACCTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAAA287730.1723813483057615No Hit
TGACTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAAAA277680.16636031278470736No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAATCG277680.16636031278470736No Hit
TGACAACTATGCCGACTAGGGATCGGGTGGTGTAAAAAAAAAAAAAAAAA261800.1568464775534298No Hit
TGACGTTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAAA256510.15367719616971076No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAATC253640.1519577561751411No Hit
TGACAGAGGTGCACAATCGACCGATCCCGAAAAAAAAAAAAAAAAAAAAC249440.1494415025245513No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAAACA243430.14584086337215973No Hit
TGACGTTTCTTTACTTATTCAATGAAGCGGAAAAAAAAAAAAAAAAAAAA233170.1396940151685761No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAACGT229180.1373035742005158No Hit
TGACAAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAT225240.1349430886330578No Hit
TGACAGAGGTGCACAATCGACCGATCCAAAAAAAAAAAAAAAAAAAAAAA218100.13066545742705515No Hit
TGACAAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAATCG214760.12866443666682423No Hit
TGACAGAGGTGCACAATCGACCGATCCTAAAAAAAAAAAAAAAAAAACCG214000.12820911457766987No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAAAT209150.12530344071925076No Hit
TGACAGAGGTGCACAATCGACCGATCCTGGAAAAAAAAAAAAAAAAAAAA206600.12377571528853551No Hit
TGACANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205730.12325449131805619No Hit
TGACATGATTAATAGGGACGGTCGGGGGCAAAAAAAAAAAAAAAAAAAAC203310.12180464992890684No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAATC196210.11755098304338603No Hit
TGACTATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAAAA192890.115561944443396No Hit
TGACTATAGATGGATACGAATAAGGCGTCAAAAAAAAAAAAAAAAAAAAT191370.11465130026508732No Hit
TGACAGCGGAGAATTTGGACAAACTTGGTCAAAAAAAAAAAAAAAAAAAA191360.11464530918496686No Hit
TGACAGAGGTGCACAATCGACCGATCCTTAAAAAAAAAAAAAAAAAAAAA189780.11369871852593548No Hit
TGACAGAGGTGCACAATCGACCGATCCTGCAAAAAAAAAAAAAAAAAAAA185080.1108829108693231No Hit
TGACACTATGCCGACTAGGGATCGGGTGGTGAAAAAAAAAAAAAAAAAAA184440.11049948174161417No Hit
TGACAGAGGTGCACAATCGACCGATCCTCAAAAAAAAAAAAAAAAAAAAC183390.10987041832896674No Hit
TGANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN181020.10845053234041964No Hit
TGACAAGAGGTGCACAATCGACCGATCCTGAAAAAAAAAAAAAAAAAATC179520.10755187032235186No Hit
TGACTTCTTTACTTATTCAATGAAGCGGAGAAAAAAAAAAAAAAAAAAAA174510.10455033918200549No Hit
TGACGCCAAGGACGTTTTCATTAATCAAGAACAAAAAAAAAAAAAAAAAA171670.10284887242779715No Hit
TGACAGAGGTGCACAATCGACCGATCCCAAAAAAAAAAAAAAAAAAAACG169390.10148290616033412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)