FastQCFastQC Report
Sat 1 Jul 2023
SRR5512735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5512735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18627547
Total Bases528.6 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCCTGGGAATACCGGGTGCTGTAGGCTT1202160.6453667785672478No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC673600.361614978075213No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC468230.25136428323063686No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA417730.22425389666175582No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT389100.2088841864148833No Hit
CTGGTCCGAAGGTAGTGAGTTATCTCAA336330.18055517454874762No Hit
AGCTTGGGCTGCAGGTCGACCCG317440.1704142794539721No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA313590.16834744800268117No Hit
TGGGGGGGCGGGGCCACCCCTCCCAGGGCG275280.14778113296399145No Hit
CCTCTAGCATCACCAGTATTAGAGGC268730.1442648353001069No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG256170.13752213321485648No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCG252020.13529424996216624No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA240590.12915817632885318No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCGC209690.11256984078472597No Hit
TCGACTGCCTCATTTGTGGTAGTGGGGG196030.10523661542767816No Hit
ACCTCTAGCATCACCAGTATTAGAGGC195100.10473735484334033No Hit
GTGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC190040.10202094779307228No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)