FastQCFastQC Report
Sat 1 Jul 2023
SRR5512736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5512736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20217806
Total Bases579 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGC884550.4375103807010513No Hit
ACCTCTAGCATCACCAGTATTAGAGGC765090.37842385073830465No Hit
CTCTAGCATCACCAGTATTAGAGGC637140.3151380520715255No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA571100.28247377583898076No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCG437460.21637362629753198No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG374540.1852525442177059No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT330530.16348460362118422No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC328390.1624261307087426No Hit
GGAGAGCGACGCTCAGACAGGC322370.15944855737561237No Hit
GCTAAGACTTCACCAGTCAAAGCGAA305890.15129732672279078No Hit
TGCTAAGACTTCACCAGTCAAAGCGA283020.13998551573795892No Hit
GTGGGGGGGCCGGGCCCCCCCTCCCCCGGCG263990.13057302063339613No Hit
TGGGGGGGCCGGGCCCCCCCTCCCCCGGCG259320.128263175539423No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCGC254300.12578021571677955No Hit
GGAGAGCGACGCTCAGACAGG252160.12472174280433793No Hit
GATCAAAAGGGACAAGCATCAAGCACGCAAC241160.11928099418898372No Hit
GTGGGGGGGCCGGGCCCCCCCTCCCCCGGCGC227980.11276198812076839No Hit
AATACAAGCTTGGGCTGCAGGTCGAC216230.10695027937254913No Hit
ATGCTAAGACTTCACCAGTCAAAGCGAA213240.10547138497619375No Hit
TGGCAATTTTTGACAGTCTCTACGGAGACTG210250.10399249057983838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)