FastQCFastQC Report
Sat 1 Jul 2023
SRR5512738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5512738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29766343
Total Bases845.5 Mbp
Sequences flagged as poor quality0
Sequence length21-42
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAGCATCACCAGTATTAGAGGC828640.27838152641055036No Hit
ACTTTGCAAGGAGAGCCAAAGCTAAGACC736030.24726920602910477No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTT634340.213106460541693No Hit
ACCTCTAGCATCACCAGTATTAGAGGC588750.19779050453056995No Hit
CTCTAGCATCACCAGTATTAGAGGC515640.17322920722911778No Hit
TGCTAAGACTTCACCAGTCAAAGCGAA419340.14087723171099653No Hit
AATACAAGCTTGGGCTGCAGGTCGACCCG386580.12987151293660762No Hit
TGGGGGGGCCGGGCCCCCCCTCCACCGGCG348580.11710541667815895No Hit
AACTTTGCAAGGAGAGCCAAAGCTAAGACC345790.11616811645286759No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)