FastQCFastQC Report
Mon 3 Jul 2023
SRR5515140.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515140.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7350285
Total Bases368 Mbp
Sequences flagged as poor quality0
Sequence length12-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT439460.5978815787415046No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT428630.583147456187073No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG386840.5262925179091695No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTG249420.339333780935025No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA165980.22581437318416903No Hit
CTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGAT155630.21173328653242696No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG154280.20989662305611279No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG119310.16232023656225575No Hit
CTGGGGTAGAAAATGTAGCCCATTTCTTGCCACCTCATGGGCTACACCTT113780.1547967187666873No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG112960.1536811157662594No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG96950.13189964742863713No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC96350.13108335255027526No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA94560.12864807282982904No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG91530.12452578369410165No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG89450.12169596144911389No Hit
ATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAG86560.11776414111833759No Hit
GTGGGTACTTGCGCTTACTTTGTAGCCTTCATCAGGGTTTGCTGAAG86440.11760088214266523No Hit
CTTTGAAGTATACTTGAGGAGGGTGACGGGCGGTGTGTACGCGCTTCAGG83800.11400918467787305No Hit
GTTTTAGTTAAATGTCCTTTGAAGTATACTTGAGGAGGGTGACGGGCGGT83090.11304323573847817No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC82700.11251264406754297No Hit
AGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTC80090.10896176134666886No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA75410.10259466129544637No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT75140.10222732860018352No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA73920.10056752901418109No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT73810.10041787495314808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)