FastQCFastQC Report
Mon 3 Jul 2023
SRR5515141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12628723
Total Bases393.5 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC11567179.15941382196759No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC8428596.674142745865912No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC4869183.8556392439678975No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC4667453.69590021097145No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2940442.3283747691670804No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2361431.8698881905953595No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1748371.3844392659495341No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1641011.2994267116318887No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG1474791.167806119431078No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1437661.138404888601959No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1374221.0881701974142595No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1351121.0698785617516515No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1287331.0193667245690639No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1271791.0070614423960365No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC960580.7606311421986214No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG923080.7309369284606211No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG851460.674224939449539No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC831550.65845929156891No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG760000.6018027317568054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC741480.5871377493987318No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC673080.5329755035406193No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC670480.5309167047214512No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA593600.4700396073300523No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG582660.461376815375553No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT561520.44463719728431766No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT556030.4402899643930744No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG502660.39802915940115247No Hit
ACGATTAAAGTCCTACGTGATCTGAG482130.3817725671867219No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC426970.33809435839237273No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC389760.30862977990727963No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG377620.2990167731131643No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC365790.28964923848594987No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC357570.28314026683458016No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC347850.27544352663369054No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC345230.2733688909005289No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT324610.25704103257312716No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC322240.25516435826488554No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG316840.2508883914866135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC301220.23851976165761177No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG290110.2297223559341669No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC288400.22836829978771406No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC287570.2277110678569797No Hit
ACGATTAAAGTCCTACGTGATCTGAGT278840.22079825489877322No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC278630.2206319673018404No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT277450.219697589376218No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC267900.21213546294427393No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG267130.21152574175552033No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG260570.2063312339656195No Hit
CGCATTGGTGGTTCAGTGGTAGAATTCTCGCC245220.1941764024755314No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG244300.19344790443182577No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA241370.19112779653176334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA227610.18023199970416645No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG227040.17978064765534885No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA200630.15886800272679985No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC199330.15783860331721583No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC193400.1531429583181134No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTG187380.14837604720603975No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC186500.14767922299032135No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG181080.1433874192980557No Hit
AACGATTAAAGTCCTACGTGATCTGAG178550.1413840496778653No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG175160.13869969275595007No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG173950.13774155945933728No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC172540.13662505702278846No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG172310.13644293251186204No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG166520.1318581459107148No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC164310.13010816691442198No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG155760.12333788618215792No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC155290.12296571870330832No Hit
TTGTGGTTCAGTGGTAGAATTCTCGCC153560.12159582564286191No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC151930.1203051171523835No Hit
ACGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACC146990.11639339939596427No Hit
ACGATTAAAGTCCTACGTGATCTGAGG144300.11426333446382503No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC136860.1083720024582058No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGC136510.10809485646331779No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC131630.10423064944887935No Hit
AATGGATTTTTGGAGCAGGG130580.10339921146421535No Hit
AGCATTGTGGTTCAGTGGTAGAATTCTCGCC130570.10339129300721854No Hit
AGCATGGGTGGTTCAGTGGTAGAATTCTCGCC130380.10324084232427935No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG128980.10213225834472735No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG127150.10068318071431291No Hit
ACGATTAAAGTCCTACGTGATCTGAGC126950.10052481157437693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)