FastQCFastQC Report
Mon 3 Jul 2023
SRR5515142.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515142.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13334921
Total Bases416.9 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC13030749.771891412030111No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC9657887.242547593645286No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC5493064.119304493817399No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC5100933.8252420093077415No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2610071.957319432188612No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2570471.9276229682950503No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1965831.4741969599969884No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1530801.147963306269306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1439651.0796089455648068No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1330920.9980711546772568No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG1314490.9857501218042461No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1238210.9285469332739203No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1224870.9185431244774528No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1120320.8401399603342232No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC937470.7030187880378144No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC930270.6976194309662577No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG902040.676449451781529No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC845110.633757035381012No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG825320.6189163025412749No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC781180.5858152440498148No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC765950.5743941040220636No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA697120.5227777502393903No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT645690.48420984271297896No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG621010.4657020465288096No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT575260.431393631803293No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG566780.42503438903012625No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG540900.4056267000006974No Hit
ACGATTAAAGTCCTACGTGATCTGAG437070.32776347156462343No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC427240.32039184934053977No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC413600.31016306733275734No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC402790.30205653261837845No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC376330.2822138953804076No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC369050.2767545454525002No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC365120.27380739638427554No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC358480.2688279893071732No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT353270.26492095453733844No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG350630.2629411902777677No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC331230.2483929226127399No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC323660.24271609858056153No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC301930.22642053897432166No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG299300.22444827382179466No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC294850.22111117118729087No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG293180.21985882031097148No Hit
CGCATTGGTGGTTCAGTGGTAGAATTCTCGCC292200.21912390782067626No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC289050.2167616891018702No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA277330.2079727356465029No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT277320.20796523653945903No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG259250.1944143501112605No Hit
ACGATTAAAGTCCTACGTGATCTGAGT250770.18805510733809372No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA249810.18733519306188615No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG236640.17745886908516367No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG228480.17133959773739943No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC221520.16612021923489462No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA218510.16386298801470214No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG201570.15115950068245623No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTG201040.15076204800913331No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC200970.15070955425982652No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC190410.14279049722154333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC187070.14028579546890454No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC181790.13632626694976294No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC177980.13346910716606422No Hit
ACGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACC168600.12643494475895284No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC167560.12565503762639463No Hit
AGCATGGGTGGTTCAGTGGTAGAATTCTCGCC166710.12501761352766919No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG165330.12398273675562083No Hit
AGCATTGTGGTTCAGTGGTAGAATTCTCGCC164110.12306784569627371No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG163390.12252790998911806No Hit
AACGATTAAAGTCCTACGTGATCTGAG163250.12242292249050446No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG162170.12161301892977094No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC157120.11782596987263741No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG154480.11584620561306662No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC148010.11099428335570943No Hit
TTGTGGTTCAGTGGTAGAATTCTCGCC145420.10905201463135777No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGA141760.10630734145331644No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT141440.10606737002791392No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGC140100.10506248968404087No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC136940.10269277185819099No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG136940.10269277185819099No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG133970.10046553706617384No Hit
ACGATTAAAGTCCTACGTGATCTGAGC133700.10026306117599047No Hit

[OK]Adapter Content

Adapter graph