FastQCFastQC Report
Tue 4 Jul 2023
SRR5515143.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515143.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12483656
Total Bases389.7 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC11625789.312800673136138No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC8782787.035422956223722No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC4910813.9337915110765627No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC4497333.6025744381293427No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2609092.090004722975385No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2364321.893932354432067No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1820861.4585951423204868No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1439751.1533079732411724No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG1321961.0589526017057824No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1296971.0389344275427006No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1276181.0222806523986243No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1253291.0039446777450451No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1143480.9159816643457653No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1121860.8986630198717427No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG907190.7267021776312965No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC871860.6984011735023778No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC834200.6682337289652968No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG759080.60805904936823No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC755480.605175278780511No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC693040.5551578800312985No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC666460.5338660405253076No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG639280.5120935725880303No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA603180.48317576197229406No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT574100.45988130400260946No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG536200.42952160809301376No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG516980.41412547734413707No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT509330.4079974648452344No Hit
ACGATTAAAGTCCTACGTGATCTGAG414870.33233052881303365No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC402370.32231743649456535No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC401810.32186884995869797No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC364320.291837583477148No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG357920.28671088021009233No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC351110.2812557475149908No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC331860.2658355853445497No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC317130.25403615735646673No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC314070.2515849523569057No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC307260.2461298196618042No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT306440.24547296080571268No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC296090.237182120366021No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG288420.23103808691940886No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG281090.22516640958385908No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC281010.2251023257930209No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC266400.2133990234911952No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT266310.21332692922650223No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC248430.19900420197416527No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG242000.1938534672855452No Hit
ACGATTAAAGTCCTACGTGATCTGAGT240950.19301236753079387No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG238710.19121802138732436No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA237870.1905451415835233No Hit
CGCATTGGTGGTTCAGTGGTAGAATTCTCGCC226810.1816855575001426No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC217520.174243827289057No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG213130.17072722926681094No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA212790.1704548731557486No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG209410.16774733299283479No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA209260.1676271758850132No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC196010.15701329802743683No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC195270.1564205229621835No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC189470.15177444812641425No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTG187530.15022041619858797No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG180690.14474125208192218No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC165630.1326774784566316No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC158460.12693396870275825No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC158340.12683784301650095No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG157940.12651742406230995No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG155330.12442669038621379No Hit
AACGATTAAAGTCCTACGTGATCTGAG154980.12414632380129667No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC154930.1241062714320228No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG146430.1172973686554644No Hit
TTGTGGTTCAGTGGTAGAATTCTCGCC140200.11230684344393982No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG138590.1110171571533211No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC138100.11062464393443716No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG138040.11057658109130851No Hit
AGCATTGTGGTTCAGTGGTAGAATTCTCGCC134560.10778893618984696No Hit
ACGATTAAAGTCCTACGTGATCTGAGC132950.10649924989922825No Hit
AGCATGGGTGGTTCAGTGGTAGAATTCTCGCC132390.10605066336336086No Hit
ACGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACC131350.1052175740824643No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGC126680.10147668279228456No Hit
GCGTTGGTGGTATAGTGGTTAGCATAGCTGC126010.10093998104401466No Hit

[OK]Adapter Content

Adapter graph