FastQCFastQC Report
Mon 3 Jul 2023
SRR5515144.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515144.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14951109
Total Bases470 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC11130147.444357472077824No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC10179946.808819332398687No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC5372113.5931180757226775No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC5138083.4365878812066715No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG3476302.3251118027431943No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC2661541.7801622608730898No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2646181.7698887754747827No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC2584081.7283533950558452No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG2267971.5169242629426352No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2192281.4662992557943362No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC2027551.3561201379777246No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC1691171.1311334831416184No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1559051.0427654563952413No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1432770.9583034944096789No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG1426310.9539827446913803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1188980.7952453560468324No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC1128010.7544657724052444No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1123430.7514024544935095No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC1113000.7444263833539037No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG874120.5846522823156463No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT734460.4912411514089022No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC725620.4853285465312306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT682720.45663502286017715No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC635470.42503201601968127No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC601880.40256545517794035No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG597000.3993014832545198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC588930.3939038903401747No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT547240.3660196711829203No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG532850.35639496708906343No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC531510.355498712503534No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG517210.34593420461318286No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG497280.33260408977019695No Hit
ACGATTAAAGTCCTACGTGATCTGAG422640.2826813716627977No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC420660.28135705518567217No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA417690.27937058046998386No Hit
ACGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACCACC403560.26991977651958793No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGG382080.2555529492828926No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCG379630.2539142748541262No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG377950.25279061238868633No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG360800.24131989138732116No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT360030.2408048794239946No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC353640.23653094897508942No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC333790.22325434186855303No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC327160.21881988821029932No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC327130.21879982280913077No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG324410.21698055976984718No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG312730.2091684302482177No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC303880.2032491369034899No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG292270.19548382665125377No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGC289460.19360436740846448No Hit
ACGATTAAAGTCCTACGTGATCTGAGT288090.1926880474217665No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC279830.1871633736333539No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC273660.1830365894596849No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG270730.18107686861222133No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCT270130.18067556058884998No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC261550.17493685585463928No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGCC261210.17470944797472884No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC258200.17269621939081575No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCT257880.17248218844501767No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC253340.16944562440150762No Hit
TTGTGGTTCAGTGGTAGAATTCTCGCC251400.1681480617926068No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT236190.15797490340014242No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGC228000.15249704888112314No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTG226110.1512329286075033No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC218080.14586208956138302No Hit
TTTGTGGTTCAGTGGTAGAATTCTCGCC215880.14439062680902132No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC214040.14315994887068242No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG206530.13813691011148405No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGC199620.13351517937565702No Hit
GGTCCCATGGTGTAATGGTTAGCACTCTGG193750.12958904921367373No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCT193690.1295489184113366No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA190300.1272815280792883No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCC189550.1267798930500741No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGC188700.12621137335029795No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC186240.12456601045447532No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA181970.12171003502148235No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG178380.11930887534831029No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA172020.11505501030057369No Hit
AATGGATTTTTGGAGCAGGG171850.11494130636061847No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC171200.11450655600196616No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGC165090.1104199026306343No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC159540.10670780341444906No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGG156180.10446047848356933No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG153880.10292213106064572No Hit
ACGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGAACC151230.10114968729075548No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC150860.10090221400967647No Hit

[OK]Adapter Content

Adapter graph