FastQCFastQC Report
Mon 3 Jul 2023
SRR5515146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12760329
Total Bases400.5 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC8400836.583552822188206No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC7570645.932950474866283No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC4660713.6524998689297115No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC4248773.329671202051295No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG2740552.147711081744052No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG2640932.069640994366211No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC2335061.8299371434702036No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC2214501.7354568209017183No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG2076251.627113219416208No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC1724131.3511642215494601No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC1618241.2681804677606667No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1468981.1512085620989867No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG1157040.9067477805627113No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1154740.904945319199842No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG1009190.7908808620843554No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT967100.7578958191438481No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG912150.7148326661483415No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC839990.6582824000854524No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC825740.6471149764241972No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC789800.6189495584322317No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG742160.5816150978552356No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC588630.4612968834894461No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG555100.43502013153422614No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG530040.41538113946748545No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT510030.399699725610523No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC509010.3989003731800332No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT488270.3826468737600731No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC450920.35337646858478333No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG449470.3522401342473223No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT430000.33698190697120745No Hit
ACGATTAAAGTCCTACGTGATCTGAG419870.32904324018604847No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC381800.2992085862362953No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG362670.2842168097703437No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC359780.2819519778839558No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGG352110.27594116107821354No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC327560.2567018452267179No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG326150.2555968580433937No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA319220.25016596358918336No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG313140.2454011961603811No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG312180.2446488644610966No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG304070.23829322895984892No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT297590.23321498998967816No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC295500.2315771011860274No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG294890.23109905708544035No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC282490.22138143930301485No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC272720.21372489690508764No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC271820.21301958593700834No Hit
TTGTGGTTCAGTGGTAGAATTCTCGCC267470.2096105829246252No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGC261110.20462638541686504No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCG254710.19961084075496802No Hit
ACGATTAAAGTCCTACGTGATCTGAGT251700.19725196740616957No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC251110.19678959688265088No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC250130.19602159160629792No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG243670.1909590262131956No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGC241250.18906252338791577No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC235570.18461122750048217No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC232120.18190753545617827No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAGCC229120.17955649889591405No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC225260.1765314985217074No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC225040.17635908917395468No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCT217260.1702620676943361No Hit
TTTGTGGTTCAGTGGTAGAATTCTCGCC212150.16625746875335268No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCT209000.16378888036507522No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGCC201220.15769185888545664No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGG195850.15348350344258366No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT189160.14824069191319442No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGG188520.1477391374470047No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCG172630.13528648046613845No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG172430.13512974469545416No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC168780.132269316880466No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG168100.13173641526013946No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCT167710.1314307805073051No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC167550.13130539189075768No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA166250.13028660938130984No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA165330.12956562483616216No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC163890.1284371272872353No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG162960.1277083059535534No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC155240.12165830520514008No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC154090.12075707452370546No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG150590.11801419853673051No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC150460.11791232028578573No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGC149430.11710513106676168No Hit
ACGATTAAAGTCCTACGTGATCTGAGC145010.11364127053463903No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA143720.11263032481372542No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC141610.11097676243300622No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC141190.11064761731456924No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGC141030.11052222869802181No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGC139950.10967585553632668No Hit
TGGGTGGTTCAGTGGTAGAATTCTCGC136970.10734049255313087No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG133740.10480920985657972No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCCGG128360.10059301762517252No Hit
TTGTGGTTCAGTGGTAGAATTCTCGC127630.1000209320621749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)