FastQCFastQC Report
Mon 3 Jul 2023
SRR5515147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13092520
Total Bases411.5 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC9284767.091652332782383No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC7218745.513636794138944No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC5447654.160887285259064No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC5423974.142800622034566No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG4158083.1759202964746285No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG2340171.7874099103915824No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1883501.4386076935532657No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG1823131.3924973954593922No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC1799661.3745711291638278No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1638981.2518445646827348No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1444621.1033933879803124No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1426891.0898513044089297No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1318921.007384369090137No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1199770.9163782067928863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1100150.8402889588864482No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT774040.591207804150767No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC772350.5899169907703024No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC744940.5689813725699865No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG744050.5683015951092685No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC731620.5588076245062066No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG699490.5342668943793861No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC665840.5085651960050471No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT597770.45657367718361325No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG590300.4508681292829799No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC587440.448683675869886No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC586270.4477900358372567No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG580350.4432683700311323No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA508270.3882140336619688No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG506030.3865031330866785No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC501190.3828063657722119No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC498400.38067537800209583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG470990.3597397598017799No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC460910.3520407072129735No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG441220.3370015856382117No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT424190.32399415849660723No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC379590.2899289059707375No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC346780.2648687953121324No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA345610.2639751552795031No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC340150.25980483512723296No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC326700.24953179372649423No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG320540.24482681714444585No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC314920.2405342898082264No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG307270.2346912588256501No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC306200.23387399828298908No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG305470.23331642800622035No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA301640.2303910935404338No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG292840.22366969842322182No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG281310.2148631432298748No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG278310.21257175853082524No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC270270.20643084753737248No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT261490.1997247283181542No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC258750.19763193029302228No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG249260.1903835166950289No Hit
ACGATTAAAGTCCTACGTGATCTGAG236420.18057639018309693No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC235910.1801868547842585No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC235430.1798202332324106No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG229310.17514580844634953No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG223810.17094493649809203No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCG210060.16044275662744836No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG206380.15763199139661424No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC204160.1559363667193176No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA189180.1444947191220636No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGC183900.14046188205173643No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC178610.1364214070324124No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGC175670.13417585002734386No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC174950.13362591769957197No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC167300.12778288671699567No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG167000.1275537482470907No Hit
ACGATTAAAGTCCTACGTGATCTGAGT165230.12620183127465148No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGCG164570.12569772664086057No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGG161500.12335287629883322No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT161310.12320775526789342No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGG161000.12297097884899164No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGG159390.12174126906050171No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCA156880.11982414386229694No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC155530.11879302074772466No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC155490.11876246895173732No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGGG153960.11759386275522207No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC152350.11636415296673215No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG151350.11560035806704896No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG147590.11272848924424023No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCG145280.11096412302597208No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCC142960.10919211885870711No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGG142550.10887896294983701No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG140040.10696183775163223No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGA139020.10618276695395538No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC137050.10467809100157953No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC135730.10366988173399773No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGG133510.10197425705670109No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC133370.10186732577074545No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA132660.10132503139197038No Hit

[OK]Adapter Content

Adapter graph