FastQCFastQC Report
Mon 3 Jul 2023
SRR5515148.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515148.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15378791
Total Bases481.6 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC11876907.722908777419499No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC8480725.514555728080316No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC6965674.529400262998568No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC6568724.271285044448553No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG4995283.2481617052992005No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG2540131.6517098125593876No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2349371.5276688525125286No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG2244931.4597571421576638No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG1928811.2542013218074164No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG1719611.1181698223221839No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1702411.1069855881388857No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1580611.027785604212971No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1543421.0036029490224556No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1428130.9286360676856847No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1289190.8382908643468788No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT926490.6024465772374434No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC893000.5806698328886841No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC872090.5670731854018953No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC867060.563802447149454No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC861790.5603756498153853No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG817170.5313616655561546No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG728670.47381487920604426No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC686260.44623793899013253No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC645420.41968188526653366No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT624220.40589666638944505No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC594510.3865778525763176No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC587160.38179854320147794No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG581470.37809864247456126No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA577650.3756147020919915No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG533080.3466332301414331No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC532080.34598298396798555No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC531740.3457619002690134No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG519120.33755579356010496No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG516590.3359106707412826No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC483080.3141209214690544No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG474580.30859382899474996No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT458320.2980208262144924No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC411840.26779738407264914No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC396940.2581087160882803No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG387040.25167127897114927No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC383790.2495579789074447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA364000.23668960713491716No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC357140.23222891838506682No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG349030.22695542191840698No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA340930.22168842791348162No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG337850.21968566969926306No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG337310.21933453676560138No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG332010.21588823204632926No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC321420.20900212506951943No Hit
ACGATTAAAGTCCTACGTGATCTGAG320330.2082933567404616No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG319370.20766912041395189No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT316470.20578340651095395No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC281450.18301178551681987No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG277020.18013119496844712No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC273400.17777730382056692No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG272970.17749769796598444No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC271420.17648981639714073No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG258850.1683162219969047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC250990.16320528707360676No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCG230630.14996627498221413No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC225440.14659149734202123No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG221930.14430913327322023No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC220230.14320371477835936No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG211120.13727997213825197No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC205350.13352805171745946No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC205300.13349553940878708No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGG201050.13073199317163486No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGC197490.1284171167941615No Hit
ACGATTAAAGTCCTACGTGATCTGAGT197190.12822204294212725No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGC195540.12714913675593875No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA194610.1265444078146325No Hit
CGCATTGGTGGTTCAGTGGTAGAATTCTCGCC189180.12301357109281218No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC187560.12196017229182711No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGG180890.11762303031493178No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCGGG177600.11548372040428925No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCC175670.11422874528953543No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC173690.11294125786610923No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCG173520.11283071601662314No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGCG172230.11199189845287578No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGGG170460.11084096272587357No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG170200.11067189872077722No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCA166910.10853258881013468No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC166820.1084740666545244No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC166540.10829199772595909No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACAGGG164890.10721909153977058No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT161680.10513180132300387No Hit
ATCCCTGGTGGTCTAGTGGTTAGGATTCGGCG159570.10375978189702949No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGG158390.10299249141236133No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC157470.10239426493278958No Hit
AGCATGGGTGGTTCAGTGGTAGAATTCTCGCC156350.10166598921852829No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA154520.10047603872111924No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG154050.10017042301959887No Hit

[OK]Adapter Content

Adapter graph