FastQCFastQC Report
Mon 3 Jul 2023
SRR5515149.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5515149.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17368471
Total Bases543.7 Mbp
Sequences flagged as poor quality0
Sequence length12-48
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC15097798.692641971765966No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC10373085.9723622188734975No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCC9121855.2519591390629605No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC8102674.66516022049379No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG5730833.2995592991461367No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC2941481.6935745236296276No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGG2762041.5902608813406776No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG2561251.4746548501592338No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGG2078441.196674134412868No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC2077261.1959947424272408No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGG2043251.176413283587254No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1838001.0582393810025075No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG1686830.9712023585726112No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1637640.9428809248666736No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC1577570.9082952667508843No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC1103520.635358172863921No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC1038970.5981931282264282No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC1025590.5904895140165188No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCC976370.5621508076329804No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCT971220.5591856646448613No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCG863500.4971652369399701No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC839800.483519821635422No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC794000.4571502004983628No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGG781960.45021809922128436No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC711050.40939124693244444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT669970.38573919373789434No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC660590.38033860320807744No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGCC650070.37428165092943416No Hit
GCATTGGTGGTTCAGTGGTAGAATTCCCGCC644630.37114953872450834No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA632150.3639641048426197No Hit
ACCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGG609750.35106717223410167No Hit
ACCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGG605610.3486835427252059No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCC601320.3462135498283067No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCG533450.3071369955363371No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGG508640.29285249116056333No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGT504490.29046310409246734No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGG446050.25681592812631576No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGC416930.24004991573524231No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC410430.236307502255092No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACC398880.22965752137882486No Hit
GCATGGGTGGTTCAGTGGTAGAATTCCCGCC395150.22750995179713862No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCTGG390570.2248729896834327No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCA389950.22451602101301837No Hit
ACGATTAAAGTCCTACGTGATCTGAG385660.22204602811611915No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCA374260.2154824106278555No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCC369490.2127360548893452No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAG368260.21202787510771673No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGG362460.20868849077158258No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGC346770.19965488038641974No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGG337370.19424277473820234No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCG332230.19128338930928349No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC321730.18523795214904062No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT320830.1847197718210198No Hit
TCCCTGGTGGTCTAGTGGCTAGGATTCGGCG309050.1779373670831474No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGG300450.1729858661709485No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGGG288280.16597891662426706No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGC250090.14399079803858383No Hit
AGCATTGGTGGTTCAGTGGTAGAATTCTCGC248730.14320776998735235No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCC248190.14289686179053987No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTG243720.1403232328280365No Hit
ACGCGGGAGACCGGGGTTCAATTCCCCGACGGGG243150.1399950519536233No Hit
ACGATTAAAGTCCTACGTGATCTGAGT234730.13514718710702858No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGC231830.13347749493896152No Hit
CGCATTGGTGGTTCAGTGGTAGAATTCTCGCC231510.1332932530445541No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCG225410.12978114193241302No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA211320.12166874101928721No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCCCC209230.12046541114643886No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGGT207060.11921602079998866No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGG203900.1173966320927156No Hit
TCCCCTGTGGTCTAGTGGTTAGGATTCGGCG202890.11681511861349222No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGCG202010.11630845340387189No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGC200370.11536421369503395No Hit
ATTGGTGGTTCAGTGGTAGAATTCTCGCC194120.11176573919488941No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCC194060.111731193839688No Hit
AGCATGGGTGGTTCAGTGGTAGAATTCTCGCC192670.11093089311085588No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCCC191040.10999241096121817No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGG187740.10809241642514185No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGG187720.1080809013067414No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGC186850.10757999365632127No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGC184220.10606575558666045No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGC184190.10604848290905976No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGA184180.10604272534985952No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGGAG183800.10582393810025074No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCACC182540.1050984856410216No Hit
ATCCCTGGTGGTCTAGTGGTTAGGATTCGGCG178960.1030372794473388No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCA175490.10103940640485853No Hit

[OK]Adapter Content

Adapter graph