FastQCFastQC Report
Tue 26 Sept 2023
SRR5564114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84174211
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA37390194.442000650294186No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA15143951.7991199228466779No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG14514461.7243357350863675No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA12803441.5210644504882855No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA12694871.508166200690613No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC12426871.4763274704172755No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG9765581.1601629387414156No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA9652641.146745527558316No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA9230681.0966161595503403No Hit
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC8939531.0620271807477946No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT8565881.017637100275285No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC7808190.9276225945260123No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7058290.8385335503768487No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG6554080.7786327810069998No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG6154930.7312132691092288No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC5595040.6646976471213969No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG5506450.654173045946341No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG4919690.5844652348449099No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC4489730.5333854569780286No Hit
TAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4199520.4989081513339044No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA4109220.4881804000515075No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA3826520.4545952916624309No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG3461470.4112269017882449No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC3369470.4002971884108305No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC3357870.39891909411541737No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG3274470.3890110713363265No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG3217910.38229167363386396No Hit
TATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA3121610.37085111495728784No Hit
CATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA2815530.33448843375555964No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT2694850.3201515010339687No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2690550.31964065573480693No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2568590.30515165743579115No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2438540.289701557166957No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2352020.27942287454289294No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA2326340.27637205889580596No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2224170.2642341369852579No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2189120.26007015379092774No Hit
CTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAG2126170.2525916162136643No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA2032430.24145518869193797No Hit
GCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1975550.23469777459511915No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA1934740.229849496302377No Hit
TGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1819280.21613270601372198No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1721500.20451632151324825No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA1681260.19973575992295314No Hit
TATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1605420.19072587446052808No Hit
ACTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1558870.1851956770940211No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1547330.18382471087254978No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1546660.18374511404686644Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA1506680.17899544077698573No Hit
CAGCACTCAAGTTGCTGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1493590.17744033264535142No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA1465820.1741412224226254No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG1364130.16206032510361162No Hit
ACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGATC1336880.15882299152171442No Hit
TTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1311630.1558232604045436No Hit
CAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1302570.15474692123933304No Hit
AAAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGATCGGA1280200.15208933767136826No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1223810.1453901361784074No Hit
TATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1198110.14233694450667317No Hit
TCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC1165810.13849966470134184No Hit
ATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1152990.13697663290244563No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1136510.1350187885931001No Hit
TTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1118150.13283759796691175No Hit
TTCGCTTGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCA1117710.13278532542467192No Hit
TTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA1102110.13093202619980604No Hit
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1099640.1306385871558689No Hit
TAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1089610.12944701079526602Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAG1086620.12909179511050003No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1051930.12497058035982067No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC1014300.12050008998599346No Hit
TTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC987540.11732096900795423No Hit
TGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG947250.11253446735604092No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA917750.10902983100132653No Hit
CATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC914250.10861402668805532No Hit
CTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC914130.10859977054017173No Hit
CAGCACTCAAGTTGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC898710.1067678555371312No Hit
ACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAATAGA893470.10614533707954804No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGATCGG892810.10606692826618833No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA887840.10547648614134322No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA880670.10462468130529909No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA876360.10411264799381369No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATCTGTAGGCACCATCAATAG874530.10389524173858902No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT865100.10277494611740406No Hit
GCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG855880.10167959875501537No Hit
CCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC855240.1016035659663029No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC855010.10157624168285936No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)