FastQCFastQC Report
Tue 26 Sept 2023
SRR5564115.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564115.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80304780
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA47779435.949761645570787No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG24811803.0897040001852942No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA20323832.5308368941425403No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG20294952.5272405951426555No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA12037671.4989979425882245No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA10779971.3423821097573518No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG10109041.2588341565719998No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA9890491.2316190891750154No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC8965001.1163719021458No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG7650950.9527390523951376No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC6995990.8711797728603452No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG6882500.8570473638057411No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA5910650.7360271704872362No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC5520470.6874397763121946No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG5287250.6583979185298807No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA4840330.6027449424554803No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA4652710.5793814515150905No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC4370840.5442814238455046No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA4335650.5398993683813094No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4288320.5340055722710404Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT4202130.5232727117862722No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC4148360.5165769708851702No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA3959940.4931138594738694No Hit
CATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA3903860.48613046446301206No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG3819580.4756354478525438No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG3781310.4708698535753414No Hit
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3651370.4546889985876308No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3420710.42596592631223196No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA3112470.38758215887024405No Hit
GCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA2876670.35821902506924247No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2853040.35527648540971035No Hit
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA2507160.3122055748113624No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA2497080.31095035687788447No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2475830.30830418811931243No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT2450730.3051785958444815No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2280420.28397064284342727No Hit
ACTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA2269340.2825908993213106No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC2178130.27123292038157626No Hit
TTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG2169810.27019686748410243No Hit
CTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAG2144400.26703267227679345No Hit
GCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG2124720.2645820086923842No Hit
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA2117370.2636667456158899No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG2092450.2605635679470139No Hit
TGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2055700.25598725256454224No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1986240.24733770517769926No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1903140.23698962876182464No Hit
TATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG1842740.22946828320804816No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAATCTGTAGGCACCATCA1817480.22632276683903502No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA1794870.22350724327991436No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1712180.2132102223553816No Hit
CCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC1657810.20643976610109632No Hit
ACATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG1653250.20587192941690394No Hit
TATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1645760.2049392327580002No Hit
TTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA1637260.20388076525457138No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1589370.19791723481466483No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA1574660.1960854634057898No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA1565630.19496099734038247No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1516200.18880569749397233No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1441720.17953103165216316No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1436020.17882123579692266No Hit
TATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1396460.17389500351037634No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1343220.16726526117125282No Hit
CGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG1202020.14968224805547067No Hit
ATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATC1183320.14735361954792728No Hit
TAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1102550.1372956877535808No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1102030.13723093444748868No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAAT1062350.13228975909030571No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATCTGTAGGCACCATCAATAG1053510.13118895288673973No Hit
CAGCACTCAAGTTGCTGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1042840.12986026485596497No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG1032980.12863244255198755No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG1021410.127191681491438No Hit
TTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1015010.1263947177241504No Hit
TCCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA988900.12314335460479439No Hit
TTGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA946800.1179008273231058No Hit
CCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC925140.11520360307319191No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA903320.11248645472909584No Hit
GGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG881110.10972074140543067No Hit
CAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG864420.10764240933105103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)