FastQCFastQC Report
Wed 27 Sept 2023
SRR5564116.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564116.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90835816
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA46087665.073732149882377No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA18973792.088800523353035No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA18375942.0229839736343647No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA15787421.7380170834816961No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG13074041.4393045139815774No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC11729711.2913089259857589No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG10138421.1161258242013261No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG8984260.9890658107810689No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA8716330.9595697362370808No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG8185660.9011489476794042No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC8167140.8991101043227266No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA7651820.842379177834435No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA6426940.7075336891342507No Hit
CATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA5177210.5699524953901444No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG4715430.5191157197288788No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4706750.5181601495163538Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC4680030.5152185785395488No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA4385040.4827435028491405No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA4132750.4549692161074438No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3969280.43697301073400385No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG3834950.42218479107404067No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT3672950.4043504161398187No Hit
TATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG3650340.4018613098604189No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA3531740.3888047859888219No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA3482330.38336530163388416No Hit
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3409140.3753079071805773No Hit
GCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA3406600.37502828179580616No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG3311140.36451921123271463No Hit
CTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAG3127400.3442915072178137No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA3040980.33477763881154543No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2961390.32601567645960267No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC2712390.2986035816532985No Hit
ACTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA2651090.29185514224917625No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2580170.2840476492224168No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG2550420.28077250938110143No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA2500910.27532201615274754No Hit
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA2379520.2619583447128388No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC2365080.2603686633915415No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2324560.2559078678833028No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2234410.24598336849861072No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA2203810.2426146532332577No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA2134270.23495908265964166No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC2085560.22959666041861723No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG2024570.2228823485220852No Hit
TATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1930720.2125505208209942No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1856990.20443367845123997No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1687230.18574501493992193No Hit
TTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA1604110.1765944393563878No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1489680.1639969855062457No Hit
TATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1463290.1610917438117141No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1456340.16032662710928913No Hit
TTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1380820.15201272590538514No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1349320.14854493077928643No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATC1335250.146995982289629No Hit
TGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAA1299620.1430735206914418No Hit
TGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1298820.1429854497041123No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT1274940.140356530732327No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAAT1256670.13834520955918972No Hit
TAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG1253280.13797200875038101No Hit
ATGAGCCCCCCTTAGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC1179290.1298265433097447No Hit
ATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATC1110750.12228106147029053No Hit
TATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1091690.12018277019716539No Hit
TGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCA1082670.1191897698150254No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG1066930.11745697313931763No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG1056640.11632416006479207No Hit
ATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCG1054650.11610508348380995No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1046410.11519795231431619No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATCTGTAGGCACCATCAATAG1009010.11108063365666249No Hit
GGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1006330.11078559584910869No Hit
AAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCGG994190.10944911861638365No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATA991580.1091617870202212No Hit
CCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC972160.1070238638027978No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAATCTGTAGGCACCATCA928080.10217115240094281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)