FastQCFastQC Report
Wed 27 Sept 2023
SRR5564117.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564117.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94030759
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA17057301.8140127955364054No Hit
TAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG11675431.2416607208285961No Hit
ATGAGCCCCCCTTAGGACCTGTAGGCACCATCAATAGATCGGAAGAGCAC5858610.6230525056168057No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA4715180.5014508071768303No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA4073150.43317208574270893No Hit
AAGCACCCAAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGATC3684200.39180796147779684No Hit
AAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCGG3665240.38979160000186747No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG3579210.380642466153017No Hit
AACAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3118900.3316893358268011No Hit
AAGGCACGCAAGTAGTCCGCCTAGCCTGTAGGCACCATCAATAGATCGGA2941110.31278169306279874No Hit
AACTCGGGCACCGAAGGTACCCTGTAGGCACCATCAATAGATCGGAAGAG2716090.28885122579942163No Hit
TGGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2685840.2856341933813381No Hit
GGGAGACAACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCG2549620.27114744442294675No Hit
ACGTTCCCTATTAGTGGGTGAACCTGTAGGCACCATCAATAGATCGGAAG2508200.26674250284420226No Hit
AAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGATCGGAAGAGC2483440.26410932192943376No Hit
AAGGCCGTCTACAACAAGGCACGCCTGTAGGCACCATCAATAGATCGGAA2431180.2585515660891347No Hit
TGGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2362410.25123800181172634No Hit
TGGGAGACGCTCAAACAGGCCTGTAGGCACCATCAATAGATCGGAAGAGC2263990.2407712140237005No Hit
GGGAGACGCTCAAACAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2237100.23791151148742723No Hit
AAAAGGTGCTGGCCTCTTCCAGCCCTGTAGGCACCATCAATAGATCGGAA2215260.23558886725566047No Hit
AACAAGGCACGCAAGTAGTCCGCCTAGCCTGTAGGCACCATCAATAGATC2188190.23271002204714736No Hit
TACACTGAAAATCAAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCA2153420.2290122958594857No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA2150580.22871026703081276No Hit
CAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG2117650.22520822149271388No Hit
AAATGCTCTATCCCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2107130.22408943864847458No Hit
AAGGCACGCAAGTAGTCCGCCTAGCAGAGCCTGTAGGCACCATCAATAGA2041150.21707258579078365No Hit
ACCACCCGATCCCTAGTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG2028530.21573047177041293No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC1982560.21084164597671706No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG1940570.20637608593587978No Hit
AAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1937780.2060793745161623No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC1919180.2041012983847126No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG1903040.202384838773874No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG1902850.20236463261984303No Hit
AACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCGGAAGAGC1902650.20234336298402103No Hit
AAAAGGTGCTGGCCTCTTCCAGCCATAAGACCCCCTGTAGGCACCATCAA1735220.1845374873556003No Hit
AGAACCGCTACGAGCCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1710520.1819106873315784No Hit
TAAACTAGAGTCAAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1705160.1813406610915477No Hit
TAAGGCACGCAAGTAGTCCGCCTAGCCTGTAGGCACCATCAATAGATCGG1661450.17669218218264088No Hit
ACGACGGAGTTTCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1637000.1740919692033965No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1633850.17375697243919938No Hit
TATGGTTTGTATTCACACTGAAAATCAAAATCCTGTAGGCACCATCAATA1590960.16919569903716294No Hit
ACAAGATTACCAAGACCTCTCGGCCCTGTAGGCACCATCAATAGATCGGA1586070.16867565644131405No Hit
GGGAGACAACAAAGGCTTAATCTCCTGTAGGCACCATCAATAGATCGGAA1579950.16802480558515964No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1577800.16779615700007272No Hit
TATGCGCGTCACTAATTAGATGACGAGGCCTGTAGGCACCATCAATAGAT1570840.16705597367346572No Hit
ATCGAAGAATCAAAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1568790.1668379599062898No Hit
TGGGAGAGCAAAGGTGCTGGCCTCTTCCAGCCCTGTAGGCACCATCAATA1552280.16508215146918043No Hit
TGGGAGAACCTACAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1485760.15800787059476995No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1476600.15703372127412052Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCAAGCACCCAAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAG1407410.1496754907614858No Hit
ATTACGATGGTCCTAGAAACCAACCTGTAGGCACCATCAATAGATCGGAA1407100.14964252282596166No Hit
AAATGCTCTATCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1404430.14935857318773743No Hit
AACAAGGCACGCAAGTAGTCCGCCTAGCAGAGCCTGTAGGCACCATCAAT1389770.1477995088819819No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA1377570.14650206109683747No Hit
TGGGAGACAACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATC1361320.14477390318629674No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC1358180.1444399699038907No Hit
AAAAGGGCAGGGACGTAATCCTGTAGGCACCATCAATAGATCGGAAGAGC1345890.14313295078262636No Hit
AAGGCCGTCTACAACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1337080.1421960233246655No Hit
TAGCCGCAAAAACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1317090.1400701232242526No Hit
TACTCGCCGTTACTAAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1307750.13907683123136333No Hit
AGAGGGCACAAAACACCATGTCTGATCCTGTAGGCACCATCAATAGATCG1295220.13774428854711254No Hit
GGGAGATGATCCTTCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1269390.1349973150806961No Hit
AAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1223340.1300999814326714No Hit
ACTGAAAATCAAAATCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1215660.12928322741710507No Hit
ACCGTTGCTAGCCTGCTATGGTTCAGCGACGCCCTGTAGGCACCATCAAT1215230.1292374977000877No Hit
TAAGGTCATTTCGACCCCGGAACCTCTAATCCTGTAGGCACCATCAATAG1214760.1291875140559059No Hit
ACTGGCGCCGAAGCTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1198220.12742851517342318No Hit
ACCCAAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGATCGGAA1192360.12680531484383745No Hit
AAGAGACCTACCAAGGCCGTCTACCTGTAGGCACCATCAATAGATCGGAA1163180.12370207497740182No Hit
TAAGCACCCAAGGCCTTTCCGCCAAGTGCCTGTAGGCACCATCAATAGAT1153190.12263965666809093No Hit
ACGACGGAGTTTCACAAGATTACCCTGTAGGCACCATCAATAGATCGGAA1125160.11965871720763202No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1109230.11796459071440656No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT1101210.11711167831794274No Hit
ATTACGCGTATGGGTTTTACACCCAAACCTGTAGGCACCATCAATAGATC1098260.11679795118956766No Hit
AAATTAAGCCGCAGGCTCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1097190.11668415863791974No Hit
TAAGGCACGCAAGTAGTCCGCCTAGCAGAGCCTGTAGGCACCATCAATAG1090100.11593015004802844No Hit
TGGGAGATGATCCTTCCGCAGGTTCCTGTAGGCACCATCAATAGATCGGA1078990.11474862177811411No Hit
AAGAGACCTACCAAGGCCGTCTACAACCTGTAGGCACCATCAATAGATCG1059220.11264611827710548No Hit
AATGCTCTATCCCCAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1019030.10837198495866655No Hit
ACGCAAAATGACATTGCAATTCGCCAGCCTGTAGGCACCATCAATAGATC1012450.10767221394012146No Hit
AAAGCGACGGTCTACAAGAGACCTACCCTGTAGGCACCATCAATAGATCG1008220.1072223611424853No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG1001320.10648855870662494No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA996220.10594618299316291No Hit
AAGTAGTCCGCCTAGCAGAGCCTGTAGGCACCATCAATAGATCGGAAGAG989650.1052474754564089No Hit
ATGCCCCCTGGAATACCAAGGGGCGCCTGTAGGCACCATCAATAGATCGG985140.1047678451686219No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT972510.10342466766646008No Hit
ACGAAAAAATCGGACCGGCCAACCGGGCCCCTGTAGGCACCATCAATAGA962550.1023654398025225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)