FastQCFastQC Report
Wed 27 Sept 2023
SRR5564118.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564118.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67272426
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA32921524.893761375574593No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA13198591.9619613539728746No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA13007341.933532172602189No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA9463211.4066996781118017No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG7821191.162614530952102No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC7624631.133396021722184No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG7218611.0730414271071478No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG7165891.065204635254272No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC6881191.0228841754569695No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG6785101.0086004628404512No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG6483280.9637351267813651No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5320750.7909258393624752Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC4759180.707448843899282No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA4375380.6503972370492481No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA3835710.5701756615704627No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC3697110.5495728666006485No Hit
CATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA3345780.49734790298777093No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT3301720.49079841419722253No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA3269100.4859494735629127No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT3057980.45456663031596334No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG2953840.4390862907188749No Hit
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2777190.41282738933779495No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2608750.38778889882758205No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG2460420.3657397460290788No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2459350.36558069126271736No Hit
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA2416660.3592348520328374No Hit
CTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAG2320180.3448931661837199No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2302290.34223382994988166No Hit
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA2283420.3394288173879741No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC2261260.3361347485818335No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG2206770.3280348474425465No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA2183820.32462334567806433No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2166600.32206360448484495No Hit
GCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA2159880.32106468109236913No Hit
TATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAG2070320.3077516484985988No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2019960.30026566902760427No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1989740.2957734867477501No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG1961150.29152360285029705No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1882940.27989773997447337No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1844370.2741643359197422No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA1657510.24638772503908216No Hit
ACTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1654420.24592839865772048No Hit
TATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA1460230.21706218830282709No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1456590.21652110479856934No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT1448980.21538988351631616No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG1426810.21209432821703203No Hit
CAGCACTCAAGTTGCTGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1416310.2105335104162885No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1416210.21051864548485288No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1398630.20790539053846518No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1379800.20510632394913184No Hit
TGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1366260.20309361223274452No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAGA1348920.20051603312180236No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1273980.18937625350392448No Hit
TATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1206360.17932458686713634No Hit
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1135420.1687794045066845No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1133450.16848656535740214No Hit
CAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1043690.15514380290076055No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1006220.1495739130918216No Hit
ATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCG953440.14172820228008426No Hit
TTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC929190.13812345640693857No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA927100.1378127793399334No Hit
TTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC887530.13193072597084576No Hit
CAGCACTCAAGTTGCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC882660.1312068038099295No Hit
TTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACA863620.12837652086458126No Hit
TTCCCGTTTAGCTCAATCGGCTGTAGGCACCATCAATAGATCGGAAGAGC846760.1258702934245303No Hit
TGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAA844110.12547637274148551No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA830140.12339974181992486No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA794290.11807066390024347No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAAT791340.11763214842289173No Hit
AAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGG786620.11693052365912894No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA785830.11681309070078727No Hit
TAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC773490.11497875816162777No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATA768770.11427713339786498No Hit
TTTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA750270.11152712108226928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)