FastQCFastQC Report
Wed 27 Sept 2023
SRR5564119.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564119.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86617747
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA19072782.201948291266454No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG13498711.5584231254594973No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10405711.2013369500363476Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGA9972861.15136451194003No Hit
AAAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATC9315691.0754943787674367No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT9090051.0494442899790501No Hit
AAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG8719481.0066620642995945No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA8243380.9516964231360117No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT6109010.7052838721376579No Hit
TTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6021750.6952097241688819No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGAATGCTGTAGGCACCATCAA5907450.6820138140974736No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA4716410.544508505860814No Hit
AAAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGATCGGA4223510.4876033083612761No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG3888630.4489414853979058No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA3685100.4254439912873744No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA3606400.41635809345168023No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGATCGG3594710.4150084855012449No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAG3568100.4119363668048304No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGAT3554790.41039973020771364No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCTGTAGGCACCATCAATAGATC3520400.40642941220810097No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA3436770.3967743469476296No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG3390240.3914024685957256No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA3172370.3662494246127182No Hit
AAAAGGCATGCCTGTTTGAGTGTCCTGTAGGCACCATCAATAGATCGGAA2552670.29470519476799595No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC2379410.2747023655556407No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2249000.25964655949778975No Hit
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2198490.2538151910139154No Hit
TTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAG2176720.251301849261907No Hit
TCTACCAAAGGCATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1987440.2294495145434803No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC1963960.22673875366441937No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1950990.22524136999314934No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG1913130.22087044125033636No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1732670.20003637360828608No Hit
AAAAAGGCATGCCTGTTTGAGTGTCTGTAGGCACCATCAATAGATCGGAA1730490.19978469308373953No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCTGTAGGCACCATCAATAGATCGGA1703640.19668486643966854No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1679910.19394524311513206No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1669390.1927307114095221No Hit
TAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1563990.18056230439704232No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1517950.17524699643827032No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCTGTAGGCACCATCAATAGATC1417390.16363736637019663No Hit
TGCTAAGCGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1385060.15990487492130223No Hit
GCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1384120.15979635212631424No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1382080.159560834571234No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC1375070.1587515316001004No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATAGA1360870.15711214469709078No Hit
CAGCACTCAAGTTGCTGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1330030.15355167342323045No Hit
TGGGAGACGCTCAAACCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1258870.14533626694307808No Hit
CAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCG1236980.14280907121724143No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1218500.1406755592476909No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA1176280.13580126945578486No Hit
CTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1168730.1349296236024241No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG1161090.13404758726869218No Hit
GGGAGACAACAAAGGCTTAATCTCAGCCTGTAGGCACCATCAATAGATCG1141190.1317501366088407No Hit
TATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1141130.13174320962192654No Hit
TGGTCCGCATGATTTATATGTATAAAAATCTGTAGGCACCATCAATAGAT1131530.13063489171566656No Hit
TACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAAGA1113700.12857642210435236No Hit
AAAAGGCATGCCTGTTTGAGTGTCTGTAGGCACCATCAATAGATCGGAAG1108800.12801071817303214No Hit
TCTACCAAAGGCATGCCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1102260.1272556765993925No Hit
CGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG1098130.12677886900013688No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1054330.12172216855282555No Hit
CTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAG1014760.11715382068296006No Hit
AAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGG999890.11543708242607603No Hit
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT963660.11125433682776349No Hit
TCCGCATGATTTATATGTATAAAAATCTGTAGGCACCATCAATAGATCGG961060.11095416739481806No Hit
CATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCA956610.11044041586535377No Hit
TGTACACGGAGTCGAGCACCCGCACTGTAGGCACCATCAATAGATCGGAA951210.10981698704308253No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG945250.1091289063429461No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC932900.10770310153645533No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA930210.10739254162313873No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA929030.10725631088049425No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT928720.10722052144810464No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC905520.10454208650797624No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC890770.10283920222492048No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)