FastQCFastQC Report
Wed 27 Sept 2023
SRR5564120.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564120.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119084969
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA25461522.1380968743418824No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG23662431.987020712916338No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA21236891.7833392558552037Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC20717651.739736775679893No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG15489631.3007208323663417No Hit
ACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC13494661.133195911567983No Hit
ATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT12899251.0831971581568787No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG12460171.0463260060973776No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG11297330.9486780821179877No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA9616350.8075200489828401No Hit
TTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT9436710.7924350217532492Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9162200.7693834139554591No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG8358630.7019047046987097No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA8198670.6884722789825809No Hit
TAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7347450.6169922251060921No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA7075370.5941446732878606No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC6811750.5720075385836478No Hit
TTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6612990.5553169350869126No Hit
AAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6318150.5305581429004697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA5967420.501106063184179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA5581520.4687006300518078No Hit
ACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGC5394400.4529874798892546No Hit
ATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA5378950.45169008693280177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG5020840.42161828164896276No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG4848170.40711855078872294No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4648800.3903767233629628No Hit
AAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4307620.36172659204370283No Hit
TTTTGGTGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4237080.35580309048071385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG4200030.3526918665948513No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3922320.3293715431038152No Hit
CCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3882590.32603526982485925No Hit
AAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3687240.30963101648873925Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3685720.3095033765344474No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATC3603230.3025763898044933No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG3499690.29388175765490604No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC3394980.28508887632997576No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA3145610.26414836619724863No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA3098570.2601982455065341No Hit
TGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3050440.2561565935328077No Hit
TCATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2982720.25046989767449157No Hit
ACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2939660.2468539921272516No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2921280.24531055636417054No Hit
TCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2892940.24293074300586162No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC2854500.2397027957407454No Hit
TGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2758370.23163040836833068No Hit
TTTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2737350.22986528215832178No Hit
TTGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAA2657170.2231322745694295No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2637730.22149982673296073No Hit
TAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2530370.2124844152245612No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2496640.20965198387044132No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2431890.20421468976491902No Hit
TTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2391620.20083307071272782No Hit
TAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2390890.2007717699452061No Hit
TGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC2309290.19391951976743596No Hit
TTTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2274890.19103082606504268No Hit
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA2260090.18978801598378045No Hit
AATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2253800.18925982169924402Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAG2240090.18810854290099366No Hit
TTTGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGA2240030.1881035044817453No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2219980.18641983271625154No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2206930.18532397652973315No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2188130.18374527183191355No Hit
GTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGGA2108380.17704837291430123No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCAT2104030.17668308751879508No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2033450.1707562270096405No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1963840.16491082094500106No Hit
CCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1906790.1601201239763517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1871520.1571583731948572Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1816550.15254234142681766No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1808450.15186215482828905No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATC1804610.15153969599639397No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG1766630.14835037661218184No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA1721000.14451865877380377No Hit
TTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1655670.1390326599488807No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1618510.13591219896106282No Hit
TAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1611300.1353067489147182No Hit
AAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1584180.1330293834144593Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1568470.1317101573079303No Hit
TCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1551710.13030275886455495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1531000.1285636644873292No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC1521420.12775919688067433No Hit
AAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1512630.12702106846078953Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAG1508020.12663394991520718No Hit
ATCAAGAGATTCCAGGTTCGACTCCTGGCGGGATCGCCACTGTAGGCACC1437810.12073815965808414No Hit
TCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1418130.11908555814462193Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1409070.11832475683811951No Hit
TGGTCCGCATGATTTATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1393500.11701728704316999No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT1384150.11623213337696717No Hit
ATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1382660.11610701263229954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1355490.11382544844933369No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA1336600.11223918612264157No Hit
TCAAAGCAGGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1323300.11112233652258834No Hit
ACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACC1276610.10720160661082258No Hit
TGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1260580.10585550893496894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1244970.10454468019385386No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1235240.10372761653907807No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)