FastQCFastQC Report
Thu 28 Sept 2023
SRR5564121.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564121.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125360299
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA27006062.154275334011448No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG24457441.950971734679733No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22326951.781022395295978Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC21496711.7147940912297919No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG16030381.278744556919093No Hit
ACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC14262441.1377158569157528No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG13166831.050318969006288No Hit
ATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT13045281.040622916829514No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG11841650.9446092658091059No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA10154020.8099869002386474No Hit
TTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT9705930.7742427289520105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9412020.7507975072714209No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG8689550.6931660237983319No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA8398270.6699305974054832No Hit
TAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7681050.6127179068071623No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA7476990.5964400260404612No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC6972570.556202406632741No Hit
AAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG6729770.5368342333006082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC6683280.5331257226819474No Hit
ACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA6236330.4974724892766888Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA5856180.4671478966399083No Hit
ACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGC5724020.4566054840057457No Hit
ATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA5540390.4419573057974279Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG5329270.42511624832675293No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG5024230.4007831857516549No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4827430.3850844357032046No Hit
AAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC4495420.35859997430286916No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG4484290.3577121334083608No Hit
TTTTGGTGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4270640.3406692576570833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4141900.33039965866705534No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4076760.32520343621707537No Hit
AAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3905070.31150771266108734Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATC3824920.30511414143962756No Hit
ATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3793470.3026053726945881No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG3765380.3003646313894002No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGTACACGGAGTCTGTAGGCAC3661560.29208290257827163No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA3372930.26905886687459163No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA3260790.26011345106954475No Hit
TGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3183350.25393605674153663No Hit
TCATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3143370.2507468492875883No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3113070.24832981612464086No Hit
ACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA3077230.24547085676622388No Hit
TCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3069320.24483987550157327No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC3021420.24101888908226043No Hit
TGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2850900.22741649650979218No Hit
TTGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAA2841840.2266937796630495No Hit
TTTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2775460.22139864232455284No Hit
TCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2730690.21782733622867315No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2645880.211062036474562No Hit
TAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2644680.21096631238890073No Hit
TAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2478500.1977101219262408No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2462880.19646411341121642No Hit
TGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC2454360.19578447240302133No Hit
TGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAG2421770.19318476577660365No Hit
TTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2413240.19250432706769469No Hit
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA2410570.19229134097709835No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2331240.1859631812141737No Hit
TTTGAACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGA2316410.18478019105554302No Hit
AATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2314770.1846493681384726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA2302640.1836817571725798No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA2300790.18353418254051868No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2240190.1787001162146239No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCAT2209250.17623203020599051No Hit
GTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGGA2180920.17397214408367037No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2144420.1710605364781397No Hit
ATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2073330.16538968210342256No Hit
CCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2011110.16042638826188504Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1968930.15706168665089096Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC1923740.15345687712502984No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATC1903650.1518542963909172No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA1829900.14597125362631752No Hit
GTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1822940.1454160539294821No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG1820970.14525890688885484No Hit
TTCTAGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1781600.14211835917845092No Hit
ATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGC1678010.13385497748374067No Hit
AAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1676430.13372894077095332Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1657940.13225399215105574No Hit
GGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1647030.1313837006722519No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC1645170.13123532833947693No Hit
TCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1613890.12874012050657282Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA1606970.12818811161259275No Hit
ACGCACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAG1606790.12817375299974357No Hit
AAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1583850.12634382756218537Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCAAGAGATTCCAGGTTCGACTCCTGGCGGGATCGCCACTGTAGGCACC1558430.12431607234759387No Hit
TCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1480750.11811953320245351Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGTCCGCATGATTTATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1461240.11656321910974382No Hit
ATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1460170.11647786513336253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT1452310.11585087237228112No Hit
TTGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1434500.11443016740092492No Hit
AACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAG1423350.11354073110498883No Hit
TTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGA1409030.11239842368276419No Hit
TCAAAGCAGGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1389950.1108764107207498No Hit
ACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACC1331140.1061851328226331No Hit
TGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1308330.10436557749435489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1290910.10297598285083862No Hit
AAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1286880.10265450946315947No Hit
AGAGCTAATACATGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1263340.10077672198277063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)