FastQCFastQC Report
Wed 27 Sept 2023
SRR5564122.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564122.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63633229
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA38275716.015050721377034No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG15143942.3798792294510154No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCAT14642312.3010477748976093No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATC14457582.272017344900099No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG14357872.256347858757883No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA13715112.1553377402866043No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG11344011.7827179569969647No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC10209121.6043693146547695No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA7469161.173782961729005No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAATCTGTAGGCACCATCA7290411.145692292308473No Hit
TTTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA6209140.9757700650394466No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA5600630.8801423545550392No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG5218320.8200621093737047No Hit
TTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG4996240.7851621045350378No Hit
ACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4502670.7075972838027754No Hit
ATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4418760.6944107771114366No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA4102160.6446568977349868No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATC3996290.6280193639081242No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG3779980.5940261180208222No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG3721530.5848406655585559No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA3721380.5848170929688323No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA3489790.5484225859416941No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA2954110.4642401535210479No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA2920810.45900703860242575No Hit
TCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2867610.45064662678048284No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC2828710.4445334685121825No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2532210.3979383161586849No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG2511390.39466644070505996No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2456570.386051444914103No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2325640.3654757170974304Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2284370.3589901119114983No Hit
ATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATC2272940.35719388057456586No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG2257590.35478161889285864No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA2241960.3523253550436675No Hit
ATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2098350.3297569576423664No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC2022150.31778208206281655No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1947240.3060099307548891No Hit
TTCCCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAA1789310.2811911367879823No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAAT1754050.27565000669697276No Hit
TTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1682980.26448131368596745Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1667650.26207219501622336No Hit
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1624300.2552597165861252No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1616570.2540449424623729No Hit
TAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1610090.2530266065863167No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1562740.2455855257635912No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1464400.2301313359408494No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA1399680.2199605492281399No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT1377200.21642780378157456No Hit
TTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1261520.19824862258679346No Hit
AAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1261270.19820933493725426Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGGA1166520.1833193157618954No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC1155240.18154665701468645No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATCTGTAGGCACCATCAATAG1131730.1778520464520196No Hit
TTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA1070880.168289432554177No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1051960.16531614323704993No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1045390.1642836638071596No Hit
AAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1010830.15885253913485986No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1007290.15829622601738472No Hit
TAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1001960.15745861332920888No Hit
TTAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT990280.1556230943427372No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG980050.1540154437235929No Hit
TCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC969170.15230564521564668No Hit
ACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACC935510.14701595608168808No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA927570.14576818033232292No Hit
ATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA927140.1457006055751155Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG899740.14139467918561857No Hit
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT898600.1412155275037198No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC876660.13776764338015912No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA864930.1359242668637796No Hit
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA864410.13584254855273806No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC855950.13451305449233136No Hit
TTTTGGTGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT841750.1322815159985045Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGG774570.12172413881432922No Hit
ACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGC763270.11994833705515714No Hit
GGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA762980.11990276338169166No Hit
TGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG747570.11748107266409503No Hit
ACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA742450.11667646160153212Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGAGTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGA740950.11644073570429689No Hit
TTTGGAAGGGATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAA728340.11445906666153938No Hit
TTGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA711440.11180322155268907No Hit
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT704080.11064659315025488No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA699590.10994098696453075No Hit
AAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT686970.10795774641579167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAGCAGGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT685680.10775502214416936Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC675420.10614265700708038No Hit
TCCCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAG670480.10536633305218567No Hit
TCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGA666470.10473615915357681No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT666240.10470001451600075No Hit
ATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA663030.10419556109591735No Hit
TGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC662810.10416098796432284No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA651780.10242761686665312No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT638040.10026836764797839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)