FastQCFastQC Report
Wed 27 Sept 2023
SRR5564123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5564123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66200307
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA40169586.067884247122903No Hit
TGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCG15472022.337152303538411No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATC15111902.282753764268797No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCAT15029242.2702674173399227No Hit
GTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG14621372.208655920583571No Hit
ATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCA14315632.1624718447302667No Hit
TCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG11952191.8054583946264782No Hit
TTGTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATC10240051.546828174074782No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA7440341.1239132168979216No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAATCTGTAGGCACCATCA7408151.1190507016833018No Hit
TTTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGA6214010.9386678524013491No Hit
TGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGA5654300.854119906120677No Hit
ATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACG5479040.8276457086520762No Hit
TTGGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGG5191270.7841761217210065No Hit
ACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4708530.7112550097388521No Hit
ATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4423440.6681902547672475No Hit
TGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCA4258890.6433338745694941No Hit
TTGGTCCGCATGATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATC4163100.6288641531526432No Hit
GCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAG3876600.5855864082322155No Hit
TTTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA3861290.5832737301354206No Hit
ATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG3798140.5737344994487714No Hit
ATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGA3615740.546181757132939No Hit
ATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA3041140.459384576570015No Hit
CATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC3012920.4551217564595282No Hit
TTTTGCTCGAATACATTAGCATGGAATAATAAAACTGTAGGCACCATCAA2948580.44540276829834036No Hit
TCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2925330.44189069999327946No Hit
CCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAG2638660.39858727543363204No Hit
AAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2626280.39671719347162543No Hit
ATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2536290.38312360092227365No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2423120.3660285140369515Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATC2391610.36126871737921096No Hit
TTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAG2381280.35970830165485485No Hit
TTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAA2373680.3585602707250285No Hit
ATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2305130.3482053338513974No Hit
ATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2149470.32469184772813814No Hit
TACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCAC2118130.3199577307096174No Hit
TTTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2032530.3070272770789416No Hit
TTCCCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAA1902590.28739896931293685No Hit
TCCGCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAAT1881400.2841980778125394No Hit
ACATTGCGCCTTTGGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1733060.26179032674274455No Hit
TTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1719820.259790336017626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA1703880.25738249219901654No Hit
CCGGAGCTAGGGTATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1695710.2561483589494532No Hit
TAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1670660.252364388582065No Hit
ACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1626150.24564085480751624No Hit
ATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGC1536500.23209862153660407No Hit
ACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATAGAT1430160.21603525192111267No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA1410370.2130458398025254No Hit
AAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG1344510.2030972454553723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGAGTGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1273920.19243415291110358No Hit
TCATATTCTAGCGTTAAAGTCTGTAGGCACCATCAATAGATCGGAAGAGC1224250.1849311665578832No Hit
GTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGGA1168920.17657319927534476No Hit
TCTACCAAAGGCATGCCTGTTTGAGTGTCATCTGTAGGCACCATCAATAG1159310.1751215443759196No Hit
TTGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAA1114360.1683315456528019No Hit
CCTACCAAAGGCATGCCTGTTTGAGTGTCATTCTGTAGGCACCATCAATA1100560.1662469631749593No Hit
ATGTACACGGAGTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1088370.16440558198619834No Hit
AAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1046590.15809443300617926No Hit
TAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1027010.15513674279486347No Hit
TCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1024730.15479233351591556No Hit
TTTTGGTGAGTCATAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1014190.15320019588428796No Hit
TTATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAG998680.15085730644723447No Hit
TTAAGTGGGAGTATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT992210.14987996958986913No Hit
TATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGA965690.14587394587158034No Hit
ACTTGGATAACCGTGGTAATTCTAGAGCTAATACATGCTCTGTAGGCACC957100.14457636880747396No Hit
ATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA944350.14265039586598896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT929610.140423820088931No Hit
TATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC929240.14036792910945262No Hit
ATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG923030.13942986699442347No Hit
TTTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA909220.13734377394956795No Hit
TATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGC900200.13598124250390559No Hit
TTGGTGAGTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA885810.13380753657230018No Hit
TTTTGGTGAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT848800.12821692805744844Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCGTGCACCATCGACCGACCCGGCTGTAGGCACCATCAATAGATCGGAA798120.12056137443592217No Hit
ACGCACATTGCGCCTTTGGGCTGTAGGCACCATCAATAGATCGGAAGAGC794020.11994204196062112No Hit
TGGAAGGGATGTATTTATTAGATCTGTAGGCACCATCAATAGATCGGAAG788210.11906440252610911No Hit
TGTGGCGTGCACCATCGACCGACCCGCTGTAGGCACCATCAATAGATCGG781590.11806440716354985No Hit
ACCGACCCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA771900.11660066772802126Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGGTGAGTCATAATAACTTTCTGTAGGCACCATCAATAGATCGGAAGA752800.11371548473332609No Hit
TTTGGAAGGGATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAA748900.11312636359828362No Hit
TTGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCA734800.11099646411005314No Hit
AAAATAGAGGTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT722520.10914148781817581Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGTATTTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT720110.10877744116805983No Hit
TCCCATATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAG713760.10781823111484967No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA707950.10694059168033768No Hit
AAAAGCAGGCAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT699490.10566265198739938Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC695990.10513395353287411No Hit
ATTTATATGTATAAAAATAGAGGTCTGTAGGCACCATCAATAGATCGGAA692760.10464604038769791No Hit
ATTCTAGCGTTAAAGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA687890.10391039425240128No Hit
TCATGATTTATATGTATAAAAATAGAGGTACTGTAGGCACCATCAATAGA679360.10262188059037249No Hit
TGGTGTTGACGCAATGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC672470.10158109991846413No Hit
TTTTGGTGAGTCATAATAACTTTTCGAATCGCATGGCCTGTAGGCACCAT668240.10094213007199497No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)