FastQCFastQC Report
Tue 4 Jul 2023
SRR5640835.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640835.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90044770
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA18097792.009865758999662No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG17421201.93472646995489No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA7180420.7974277684311927No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC6014400.6679344064069462No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA5688770.6317712844399513No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG4402620.48893678111455Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG4196280.46602151352044097No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA3108500.3452171625292618No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA3017750.33513884260018656No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC2854230.31697898723046325No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2541910.2822940188530661No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG2388630.2652713755612902No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2381060.2644306826481982TruSeq Adapter, Index 6 (100% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG2138510.23749408211048792No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA1944500.21594813335632929No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1773150.19691871054809737No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1630430.1810688172117048TruSeq Adapter, Index 6 (100% over 49bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA1585620.17609240381201485No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA1274980.14159400929115593No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC1246540.13843558043404408No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGAGATCGGAAGAGCA1228520.13643435371093734No Hit
GATGTCGATGACAGACGTGCTCAGCGCTGAGGAGATCGGAAGAGCACACG1200870.13336365898874528No Hit
CACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGAGATCGGAAGAGC1173140.13028407979719422No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGG1119170.12429039465590283No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC1113140.1236207277779709No Hit
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCG1099180.1220703878748316No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGGAG1061970.11793799906424327No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGAGATCGGAAG1006980.11183103693862509No Hit
TCCAAGTTGCAGGATGTCGATGACAGACGTGAGATCGGAAGAGCACACGT1001420.11121356631817705No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA996790.11069937765402699No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG984230.10930451596466957No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT931220.10341744445568576No Hit
GTGGGGGGAACTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC916110.10173939030551137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGGGGGGAACTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA902580.1002368044251765Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)