FastQCFastQC Report
Mon 10 Jul 2023
SRR5640836.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640836.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97877358
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA13178131.346392083856616No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG8888380.9081140093707883No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG7058110.7211177481925902Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA6064470.6195988657560617No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA5297880.5412773810261613No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG4677480.47789193492533794No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA4626720.47270585297163414No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC4362670.44572821428220405No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT3615010.36934078257404535TruSeq Adapter, Index 12 (100% over 35bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC3355960.34287398726066964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG2588380.2644513555423104TruSeq Adapter, Index 12 (100% over 49bp)
CGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGT2378930.24305212651939379TruSeq Adapter, Index 12 (100% over 38bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2363210.24144603494507894Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA2349240.24001873855238307No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG1970820.20135606847908583No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1891640.1932663527758892No Hit
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCG1806800.18459836237099903No Hit
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1719440.17567290690457746Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1676000.17123469965341728No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCT1671660.1707912876030021TruSeq Adapter, Index 12 (100% over 35bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1509640.15423791884533702No Hit
GTGGGGGGAACTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1471480.15033916219929028Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGG1439200.1470411573634834No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGGAG1428370.14593467061094967No Hit
GTGGGGGGAACTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1383290.14132890673244367Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA1326400.1355165307996973No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG1269210.12967350426438767No Hit
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTT1206740.12329102712396467TruSeq Adapter, Index 12 (100% over 36bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGAGAT1190490.12163078615178803No Hit
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGAT1098630.11224557164691758No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1011530.10334668003605084No Hit
TGGGGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACTCCA991440.10129411135106445Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)