FastQCFastQC Report
Fri 7 Jul 2023
SRR5640837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101459169
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG13936261.373583101198079No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC10024620.9880447571968582TruSeq Adapter, Index 5 (100% over 35bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA9588160.9450264667553112No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG9342670.9208305264160009Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC5843370.5759331618416863No Hit
CGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT3732630.3678947932246518TruSeq Adapter, Index 5 (100% over 38bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA3566410.3515118480814681No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3303400.32558910471659785No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA3147280.3102016339203409No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA3140460.30952944233162405No Hit
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2888320.2846780659124066Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA2700080.2661247895692897No Hit
GTGGGGGGAACTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC2663610.26253024012053555Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA2609290.25717636224676743No Hit
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA2560070.252325149637289TruSeq Adapter, Index 5 (100% over 36bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG2514250.24780904720400382No Hit
CTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGC2388370.23540208573953528No Hit
CGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCCCGTGGATCGC2370140.23360530382424086No Hit
ACCCCCCGTCCTTTCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2244140.22118651494178904Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC2159920.2128856387538518No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2152580.2121621950205407No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC2119350.20888698585733537Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG2059490.2029870755200055No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1947660.19196490757774687TruSeq Adapter, Index 5 (100% over 35bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA1729930.17050504326523708No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1644010.16203661198920324No Hit
GTGGGGGGAACTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1547840.15255792209376365Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1546030.15237952520584908Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGG1501880.14802802100616458No Hit
CGGGGTCGGGGCACGCCGGAGATCGGAAGAGCACACGTCTGAACTCCAGT1451800.14309204523447258Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1432370.1411769891393453No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG1359360.13398099091468016No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1300160.12814613137625835No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC1266370.12481572759579768Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA1247010.12290757082782731No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT1246780.12288490161002599No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGGAG1222740.1205154755407074No Hit
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1208710.11913265325482805Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAG1191950.11748075720982891TruSeq Adapter, Index 5 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1167550.11507584888656047No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG1148120.11316079279143317No Hit
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA1146710.11302182063012954Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1065780.10504521281856745No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1040590.10256244066024235No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC1029890.10150782922339922No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGGG1025350.10106035857636485No Hit
CGGCGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGT1016730.10021075571789868TruSeq Adapter, Index 5 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)