FastQCFastQC Report
Tue 4 Jul 2023
SRR5640838.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640838.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57848964
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG29759045.144264986318511TruSeq Adapter, Index 4 (100% over 49bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA15879332.74496359174211No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG6589231.1390402773678021Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG4045360.6992968793702166TruSeq Adapter, Index 4 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG3567750.6167353316819987No Hit
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT3061340.5291953024430999Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2419580.4182581385554286No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG1922950.33240871867644856TruSeq Adapter, Index 4 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1754740.3033312748695033Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT1474670.25491727042855944Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1248840.21587940624139784Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTC1178540.20372707106734012TruSeq Adapter, Index 4 (100% over 50bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA1135430.19627490649616475No Hit
CGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACC1048350.18122191436306448TruSeq Adapter, Index 4 (100% over 38bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT1037710.1793826420123963No Hit
GGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT985960.17043693297601664Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT983810.1700652754991429No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC789200.13642422360407352No Hit
GGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC725790.1254629209954391Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTC673180.11636854896830996Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC625630.1081488684914046Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA615630.10642022906408488TruSeq Adapter, Index 4 (100% over 36bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA590510.1020778868226577No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC585840.10127061221009939No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT579810.1002282426354256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)