FastQCFastQC Report
Tue 4 Jul 2023
SRR5640839.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640839.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56022467
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA33351355.953209807772299No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG13680902.4420381201706096TruSeq Adapter, Index 5 (100% over 49bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG10210301.8225366619431451No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA5576900.9954756187370328No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG4907970.8760717374334837Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC4555020.8130702276998976No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC2172750.38783547322184153TruSeq Adapter, Index 5 (100% over 35bp)
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1761200.3143738743243849TruSeq Adapter, Index 5 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG1284970.2293669073873523No Hit
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1214540.2167951654110484Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1169080.2086805638173699Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA1140840.2036397290394227Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1005220.17943158411785043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT910870.16259012656475838Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC858790.15329385619522967No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT821400.14661974810927195No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA818960.14618420856046915No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA787330.14053825940939016No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC769830.13741451264543564No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA753850.13456208560040744No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA674490.1203963402754113No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA642800.11473968113542732No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT640740.1143719715163561No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG622340.11108757491882677No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC612130.10926509180682814No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT610980.10905981701948256No Hit
GGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC587850.10493111629661007Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)