FastQCFastQC Report
Tue 4 Jul 2023
SRR5640840.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640840.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107218164
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG37117763.4618910280910984No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA34612273.2282095410624643No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA11337251.0574001248519795No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC9750390.9093972174341652No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG7399310.6901172081252949Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA5598780.5221857744178495No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT5074540.47329107407584403TruSeq Adapter, Index 18 (100% over 35bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA5061130.4720403531625481No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA4974140.46392698908740876No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG4885980.4557045017111093No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG4846300.4520036362495444No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT4304050.40142918321190424TruSeq Adapter, Index 18 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA4237250.3951988955901166No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3416730.3186708177543499No Hit
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG3248300.30296172577624064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATG3095620.2887216013137476TruSeq Adapter, Index 19 (97% over 49bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC3054260.2848640459838503No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG2872990.26795739572634353No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA2762260.2576298545832216No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG2389990.22290905858078303No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC2199280.2051219604916943Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCG1973880.18409940315709938No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC1965640.18333087666004055No Hit
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTG1825890.17029670457703416TruSeq Adapter, Index 19 (100% over 36bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1714820.15993745238913065No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA1662030.15501384634790052No Hit
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT1497150.13963585498442224Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA1471190.13721462344757182TruSeq Adapter, Index 19 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1355580.12643193554405577No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1331470.1241832493979285No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA1279330.11932026741289845No Hit
CTTCGTGACCACAGCTGAACGTGAGATTGTGAGATCGGAAGAGCACACGT1239460.11560168107336737No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1228090.11454122642876072No Hit
GTGGGGGGAACTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1221180.113896746077465Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1213310.1131627286585508No Hit
TCCAAGTTGCAGGATGTCGATGACAGACGTGAGATCGGAAGAGCACACGT1195350.1114876393518546No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAAG1158830.10808150007120061No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAA1117540.1042304734858172No Hit
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1097470.10235858916591782Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1080380.10076464282675088No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)