FastQCFastQC Report
Mon 10 Jul 2023
SRR5640841.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640841.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79103076
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG40570025.128753779435834No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA23204132.933404258514549No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC12744081.611072621246739No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA12303941.5554312957437963No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA6267280.7922928306858762No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA6043790.7640398206512222No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG5616010.7099610133997823No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA5170120.653592788224822No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG4704150.5946861029778412No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC3988930.5042698971655667No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCAGATCGGAAG3777760.47757434868904464No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG3771910.4768348072836004No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA3521180.445138189063596No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG3305660.4178927251830258No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA3241610.40979569492341866No Hit
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATCG2289290.2894059391571574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC2026170.25614301016562235No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1974910.24966285761125143No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA1916680.2423015762370606No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1850480.233932748708786No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG1761040.22262598233221678TruSeq Adapter, Index 10 (100% over 49bp)
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1694210.21417751188335582Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1574450.1990377719319031No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA1549070.19582929998828366No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA1424740.18011183281924462No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGG1383620.17491355203431028No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1312030.16586333507435286No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCAGATC1226500.15505086047475575No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1196330.15123684950001187No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGA1169890.14789437518207257No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1141680.14432814218248605Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGAT1092150.13806669161639176No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAAGATCGGAAG1055790.1334701573425539No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAAGATCGGA1023290.12936159397897498No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT979990.1238877234053452No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAAAGATCGGA951030.12022667740506071No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA911490.11522813600826345No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGGAG893370.11293745391140037No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC859170.10861398107957268No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG849540.10739658215061067No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCAGATCGGAA846340.10699204668096597No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)