FastQCFastQC Report
Mon 3 Jul 2023
SRR5640842.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640842.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109655193
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG19424011.7713716485821152No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA9952700.9076359931261988No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGA7965590.7264215931843738No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC7572520.6905755936246449No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATC6249110.569887282948834No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA6048640.5516054310350811No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT5352260.48809909075623986No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG5118260.4667594721209418Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG4821360.4396836910405146TruSeq Adapter, Index 13 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC4413580.40249621374520766No Hit
CATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAA4132060.3768230110178184No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA4055810.36986939597105994No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA4044480.36883615717132523No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC3807840.3472557838642443Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGGTCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTC3599910.328293617612802TruSeq Adapter, Index 14 (100% over 38bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAA2909050.26529067346587043No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG2780820.25359674484363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2572950.2346400502892736No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCG2437400.2222785746225443No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG2392360.2181711540100066TruSeq Adapter, Index 14 (97% over 49bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA2262380.2063176342227586Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAA2085460.19018342341525038Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1995740.18200141237269082No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT1956560.1784283941755499No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCAGATC1945580.17742707360881668No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTAGATCGG1817820.1657760066137497No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG1804550.16456584960823514No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA1680930.15329232971210036No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1644680.14998651272265784No Hit
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCGGAGA1492850.13614038324660102No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1486220.13553576071860088TruSeq Adapter, Index 13 (100% over 35bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA1324540.12079136097093003No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTAGATCGGAAGAGCACACGTC1303960.11891456887044101No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTAGATCGGAA1297040.11828349980652535No Hit
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGT1278590.11660095295258838TruSeq Adapter, Index 13 (100% over 36bp)
GTGGGGGGAACTCCGCGTCGGTAGATCGGAAGAGCACACGTCTGAACTCC1264670.11533151922864246Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCCCGCCTCCCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGAGATCGG1249110.11391252578434657No Hit
GCTCTTCTGGAACGGAAAAAACCTTTAATAGTGAATAATTAACAGATCGG1230160.11218438145469316No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1173690.10703460254727745No Hit
GTGGGGGGAACTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA1154910.10532196135936762Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)