FastQCFastQC Report
Mon 3 Jul 2023
SRR5640843.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640843.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50941583
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA37103287.283495685636625No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG12360582.4264224376380295TruSeq Adapter, Index 6 (100% over 49bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC8440521.6569017888588191No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG4345900.8531144389447026No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA3150160.6183867509574644No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG2983620.585694402154719Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC2147270.4215161511569046TruSeq Adapter, Index 6 (100% over 35bp)
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC1876250.3683140353137436TruSeq Adapter, Index 6 (100% over 35bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG1282210.2517020328952086Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1281700.2516019182207196No Hit
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1165610.22881307006105406Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC796350.15632611966534293Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGGCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGT742520.14575911392466936Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCC735970.14447332741897714TruSeq Adapter, Index 6 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT726450.1426045201618489No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC717340.14081619725087854No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT678830.13325655781054152No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT669640.1314525306369062No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG658390.12924411869964858No Hit
TGGGGGTGGGGGCCGGGCCGCCAGATCGGAAGAGCACACGTCTGAACTCC629090.12349243249861316Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGT615610.12084626423957026Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC592390.11628810200107052No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC588560.11553626042598637TruSeq Adapter, Index 6 (100% over 50bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGC525880.10323197062800345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)