FastQCFastQC Report
Tue 4 Jul 2023
SRR5640844.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640844.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59405061
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA885129914.899907265476925No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG40861596.878469496058594TruSeq Adapter, Index 7 (100% over 49bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC26676574.490622440401164No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG5003950.8423440555005912No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA3942690.6636959770144837No Hit
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA3926900.6610379543251375TruSeq Adapter, Index 7 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA2882860.48528861875926693No Hit
CCCCCGCCGGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCA1314980.22135824420750952TruSeq Adapter, Index 7 (100% over 35bp)
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC1244830.2095494860277982Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1144140.19259975172822397Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG1090960.18364765251230028Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC1058650.17820872198077536No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1000830.16847554453315014Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCGGGGCGGACTGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC929650.15649340045286714Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC907670.15279337900183285TruSeq Adapter, Index 7 (100% over 50bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC860210.14480416070947222No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGAGATCGGAAGAGC768770.12941153279852705No Hit
TGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTCTGAACTCCA752050.12659695779118887Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGTCGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG700350.11789399559744582TruSeq Adapter, Index 7 (100% over 36bp)
GCGGGGCGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG679540.11439092706259488TruSeq Adapter, Index 7 (100% over 36bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGAGATCGGAAGA672670.11323445993936443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)