FastQCFastQC Report
Mon 3 Jul 2023
SRR5640845.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5640845.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52771932
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA621428311.77573525259602No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC18015793.4138962355973628No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCA3919670.7427565850725344No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTAGATCGGAAG3568600.6762306902085753No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA2813160.5330788344076545No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2495500.4728839565699433TruSeq Adapter, Index 8 (100% over 49bp)
GCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC1682310.3187887834009943TruSeq Adapter, Index 5 (100% over 35bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATAGATCGGAAGAGCAC1134550.2149911812969061No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT931700.176552186870854No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT875500.165902586246037No Hit
TGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAG787460.14921947523164397Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA786930.14911904305493306Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG739030.1400422482163435No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAGATCGGAAGAGCAC728420.13803170973539494No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC719680.13637552629303015Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTTGTCTGCTAAAGAAACAAAGACGCTTCTGAGATCGGAAGAGCACACGT601210.11392609237804673No Hit
GGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA573460.10866761520120204Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT564550.10697921766442055No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAAAGATCGGAAGAGCA561590.10641831343222379No Hit
TGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTCTGAACTCCA537550.10186286149235545Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)