FastQCFastQC Report
Mon 3 Jul 2023
SRR5660304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5660304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12091266
Total Bases266.6 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGG2224351.8396336661520802No Hit
TTGTCGCTAAGATTCGA1174800.9716104169737064No Hit
ACCACGTTCCCGTGG669960.5540858996899084No Hit
ACCGGTAGACTTGAAC597150.4938688802314001No Hit
CTACAGTCCGACGATC377740.3124073194651412Illumina DpnII expression Sequencing Primer (100% over 16bp)
GGTGAGGGACGACGATT307520.25433234203928684No Hit
TCTACAGTCCGACGATC303100.25067681084842564Illumina DpnII expression Sequencing Primer (100% over 17bp)
TCCGTTGTAGTCTAGC266310.22024988946566884No Hit
CCGGTAGACTTGAAC234240.19372661225052862No Hit
TACAGTCCGACGATC233420.1930484367807308Illumina DpnII expression Sequencing Primer (100% over 15bp)
CCCGGCTGGTGCACCA217990.1802871593429505No Hit
GACCGGTAGACTTGAAC208200.17219040586816964No Hit
CGTGGAGACCTGGGC151980.12569403402422874No Hit
GGTGAGGGACGACGATC142340.11772133703782549No Hit
CTCGTGTTGCATCCCT136450.11285005226086334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)