FastQCFastQC Report
Mon 3 Jul 2023
SRR5660305.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5660305.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12656242
Total Bases275.1 Mbp
Sequences flagged as poor quality0
Sequence length15-44
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCACGTTCCCGTGG3857333.047768840071168No Hit
ACCACGTTCCCGTGG1204050.9513487494945181No Hit
TCCGTTGTAGTCTAGC386710.3055488351123501No Hit
CTACAGTCCGACGATC297500.23506187697738398Illumina DpnII expression Sequencing Primer (100% over 16bp)
ACCGGTAGACTTGAAC276510.2184771751361897No Hit
CGTGGAGACCTGGGC253680.2004386452155387No Hit
TACAGTCCGACGATC139380.11012747701884967Illumina DpnII expression Sequencing Primer (100% over 15bp)
TCCGTCGTAGTCTAGC130910.10343512710961121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)